人参皂苷Re酶转化的F1皂苷等产品的分离提纯  

Separation of ginsenoside F1 in enzymatic products from ginsenoside Re

作　　者：宋建国[1] 肖永坤[2] 王东明[2] 鱼红闪[2] 金凤燮[2] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034 [2]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2012年第3期157-160,共4页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅创新团队项目(LT2010009;2009T007)

摘　　要：利用大孔吸附树脂和硅胶层析相结合的方法,对人参皂苷Re的酶解产物进行分离提纯。结果表明,65.0g的Re酶解产物经大孔吸附树脂AB-8和D-296脱糖、脱色处理后,共得干燥粗产品37.0g,得率为56.9%。采用硅胶层析柱进一步分离纯化后,共得到纯度为96.0%的Rg1皂苷11.2g,在粗产品中的得率为30.2%;得到纯度为98.0%的稀有人参皂苷F1皂苷4.75g,在粗产品中的得率为12.8%。Re经人参皂苷酶转化得到Rg1皂苷的得率为17.2%,F1皂苷的得率为7.31%。Ginsenoside Rg1 and F1,the hydrolysates of ginsenoside Re were separated on macroporous absorption resin and silica gel column,respectively.37.0 g dry crude product was obtained after desugarization and decoloration from 65.0 g hydrolysate by resin of AB-8 and D-296,and the yield was 56.9%.After further purification by silica gel column,11.2 g Rg1 was obtained with a yield of 30.2%,and the purity was 96.0% based on HPLC.4.75 g F1 was obtained with a yield of 12.8%,and the purity was 98.0%.The yield of enzyme transformation was 17.2% from ginsenoside Re to Rg1 and 7.31% from Re to F1.

关 键 词：人参皂苷F1 大孔吸附树脂 硅胶层析 高效液相色谱 

分 类 号：TS201.2[轻工技术与工程—食品科学] Q946.83[轻工技术与工程—食品科学与工程]