壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响  

Effects of Chito-Oligosaccharides on Growth Performance,Serum Biochemical Indices and Non-Specific Immunity Function of Rainbow Trout(Oncorhynchus mykiss)

作　　者：刘含亮[1] 孙敏敏[1] 王红卫[1] 万文菊[2] 王纪亭[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271000 [2]泰山医学院,泰安271000

出　　处：《动物营养学报》2012年第3期479-486,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省中青年科学家科研奖励基金(2006BS06018)

摘　　要：本文旨在研究壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响。选用体重约为60 g的虹鳟480尾,随机分为4组(每组4个重复,每个重复30尾),分别饲喂在基础饲料中添加0、100、200和400 mg/kg壳寡糖的试验饲料。试验期为49 d。结果表明:1)与对照组相比,100、200和400 mg/kg壳寡糖组的特定生长率分别提高了5.60%(P<0.05)、15.89%(P<0.05)和18.69%(P<0.05),增重率分别提高了7.53%(P<0.05)、21.21%(P<0.05)和26.02%(P<0.05),蛋白质效率分别提高了0.60%(P>0.05)、7.02%(P>0.05)和9.79%(P<0.05),饲料系数分别降低了0.60%(P>0.05)、6.55%(P>0.05)和8.93%(P<0.05);2)饲料中添加壳寡糖提高了虹鳟血清总蛋白、白蛋白、球蛋白含量(P>0.05),降低了血清甘油三酯、胆固醇、葡萄糖含量以及谷丙转氨酶和谷草转氨酶活性(P>0.05);3)饲料中添加200和400 mg/kg壳寡糖可显著或极显著提高虹鳟的白细胞吞噬百分率(P<0.05)、吞噬指数(P<0.05),血清杀菌百分率(P<0.01)以及血清、肝脏和鳃中溶菌酶活性(P<0.05);4)虹鳟全鱼水分、粗蛋白质、粗脂肪、灰分含量在各组间差异不显著(P>0.05)。由此得出,在本试验条件下,饲料中添加壳寡糖可提高虹鳟的生长性能和非特异性免疫功能,建议添加量为200 mg/kg。This experiment was conducted to evaluate the effects of chito-oligosaccharides on growth performance,serum biochemical indices and non-specific immunity function of rainbow trout(Oncorhynchus mykiss).A total of 480 fish with the body weight about 60 g were randomly allotted to 4 groups with 4 replicates each and each replicate contained 30 fish.The fish in the 4 groups were fed a basal diet supplemented with 0,100,200 or 400 mg/kg chito-oligosaccharides,respectively.The experiment lasted for 49 days.The results showed as follows: 1) compared with the control group,the specific growth rate in the 100,200 and 400 mg/kg chito-oligosaccharides groups was increased by 5.60%(P<0.05),15.89%(P<0.05) and 18.69%(P<0.05),the weight gain rate was increased by 7.53%(P<0.05),21.21%(P<0.05) and 26.02%(P<0.05),and the protein efficiency rate was increased by 0.60%(P>0.05),7.02%(P>0.05) and 9.79%(P<0.05),while the feed conversion rate was decreased by 0.60%(P>0.05),6.55%(P>0.05) and 8.93%(P<0.05),respectively.2) Dietary supplementation of chito-oligosaccharides improved the contents of total protein,albumin,globulin in serum(P>0.05),while decreased the contents of triglyceride,cholesterol and glucose,and activities of glutamic pyruvic transaminase and glutamic oxaloacetic transaminase in serum(P>0.05).3) Dietary supplementation of 200 and 400 mg/kg chito-oligosaccharides significantly improved the phagocytic percentage(P<0.05) and phagocytic index of leucocyte(P<0.05),serum bactericidal percentage(P<0.01) and lysozyme activity in serum,liver and gill(P<0.05).4) No significant differences were found in the contents of moisture,crude protein,crude lipid and ash of whole body among all groups(P>0.05).It is concluded that dietary supplementation of chito-oligosaccharides can improve the growth performance and non-specific immunity function of rainbow trout,and the supplemental level of chito-oligosaccharides in diets is suggested to be 200 mg/kg.

关 键 词：虹鳟 壳寡糖 生长性能 血清生化指标 非特异性免疫功能 

分 类 号：S963[农业科学—水产养殖]