重度间歇性低氧对大鼠认知功能及海马超微结构的影响  

Effects of intermittent serious hypoxia on the cognitive function and hippocampus ultra-microstructure in rats

作　　者：李琳[1,2] 王红阳[2] 陈宝元[1] 赵雅宁[2] 杨林[2] 韩晓庆[2] 张盼盼[2] 

机构地区：[1]天津医科大学总医院,天津022300 [2]河北联合大学附属医院呼吸科,河北唐山063000

出　　处：《西安交通大学学报（医学版）》2011年第6期687-689,727,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：河北省科技厅资助项目(No.09276103D-11)~~

摘　　要：目的通过模拟阻塞性睡眠呼吸暂停低通气综合征(OSAHS)的发病特征,建立大鼠间歇性低氧(IH)模型,观察重度间歇低氧(50mL/L)条件下不同暴露时间对大鼠学习记忆功能的影响和海马CA1区超微结构的改变。方法成年雄性Wistar大鼠48只分为对照(UC)组和50mL/L间歇性低氧(50mL/L IH)组。UC组放入舱内仅给予压缩空气,50mL/L IH组大鼠每日放入自制低氧舱内予间歇低氧暴露8h,分别干预2、4、6、8周。采用Morris水迷宫检测各组大鼠学习记忆功能,电镜观察海马区神经细胞超微结构。结果与UC组比较,50mL/L IH组中随低氧时间延长,神经细胞核、线粒体、粗面内质网、突触等超微结构损伤明显,水迷宫检测动物逃避潜伏期时间随缺氧时间增加而延长[从2周(49.17±8.87)s到8周的(68.42±7.91)s],均长于UC组[2周和8周分别为(25.66±2.97)s,(25.29±3.07)s];50mL/L IH组的穿台次数也随缺氧时间增加而减少(从2周7.68±1.6到8周的2.87±0.92),均少于UC组(2周和8周分别为11.57±2.18,10.65±1.26)。结论间歇性低氧可造成认知功能障碍,与海马区神经细胞超微结构损伤有关。Objective We set up an intermittent hypoxia(IH) animal model by simulating the features of obstructive sleep apnea-hypopnea syndrome(OSAHS) to observe the effects on cognitive function and hippocampus ultra-structure in rats exposed to 50 mL/L IH.Methods Totally 48 mature male Wistar rats were randomly assigned into two groups: 50 mL/L IH(50 mL/L IH) group and unhandled control(UC) group.The compressed air was given to UC group while rats in IH groups suffered from 8-hour IH every day for 2,4,6 or 8 weeks,respectively.The cognitive function of rats in 50 mL/L IH and UC groups was assessed with the Morris water maze(MWM) test,and the hippocampus ultra-microstructure was also observed.Results Compared with that in UC group,the synaptic ultra-structure of nerve cell nucleus,mitochondria and rough endoplasmic reticulum was significantly injured in 50 mL/L IH group in a time-dependant manner.MWM test showed that with prolonged hypoxia,the escaping latency was prolonged [(49.17±8.87)s at week 2 and(68.42±7.91)s at week 8],all longer than those in UC group [(25.66±2.97)s at week 2 and(25.29±3.07)s at week 8,respectively].The frequency of crossing the platform was decreased in 50 mL/L IH group(7.68±1.6 at week 2 and 2.87±0.92 at week 8,respectively),all lower than those in UC group(11.57±2.18 at week 2 and 10.65±1.26 at week 8,respectively).Conclusion Intermittent hypoxia can cause cognitive dysfunction.This damage is related to the neuronal ultra-structure injury from prolonged intermittent hypoxia.

关 键 词：重度间歇性低氧 认知功能 水迷宫 海马超微结构 电镜 阻塞性睡眠呼吸暂停低通气综合征(osahs) 

分 类 号：R364.4[医药卫生—病理学]