慢性阻塞性肺疾病患者的血清差异表达蛋白分析  

Analysis of differentially expressed protein from COPD patient serum

作　　者：杨娟[1] 杨栓盈[2] 倪磊[1] 宋土生[1] 于林[1] 黄辰[1] 

机构地区：[1]西安交通大学医学院遗传与分子生物学系/环境与疾病相关教育部重点实验室,陕西西安710061 [2]西安交通大学医学院第二附属医院呼吸科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2012年第1期61-64,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30872483);中国博士后科学基金(No.20090461301)~~

摘　　要：目的应用液体芯片-飞行时间质谱技术从慢性阻塞性肺疾病(COPD)患者的血清中找出差异表达蛋白,筛选出其蛋白多肽(m/z)的疾病标志物。方法应用液体蛋白芯片(MB-WCX)结合飞行时间质谱(MALDI-TOF-MS)技术对16例病理确诊的COPD患者和32例正常对照人群进行血清蛋白组分析。采用ClinProTools软件进行蛋白多肽的组间差异对比,共获得115个蛋白多肽峰图,其中24个蛋白多肽峰图具有极显著差异(P<0.001),其中10个蛋白多肽在COPD患者中表达下调,其余14个蛋白多肽在COPD患者中表达上调。两组数据中最大差异的两个蛋白多肽峰分别是m/z:1 012.92和m/z:4 152.85。结论利用液体芯片-飞行时间质谱技术可以从COPD患者的血清中获得稳定丰富的蛋白多肽图谱,并且能够筛选出潜在的疾病标志物。Objective To screen potential specific biomarkers in serum of chronic obstructive pulmonary disease(COPD) patients using pre-fractionated magnetic bead based sample fractionation method combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) technology.Methods We compared serum proteomic profiling of 16 COPD patients and 32 healthy controls by MB-WCX-MALDI-TOF-MS.Results By analyzing the spectra(screened from two groups) using the ClinProTools software,24 of all 115 peaks generated were found to be very significantly differently expressed(P<0.001) between the two groups.And 14 peaks were up-expressed while the other 10 peaks were down-expressed in COPD patients.The two max differentially expressed peaks were m/z:1012.92 and m/z:4152.85.Conclusion We were able to identify proteomic patterns that could clearly distinguish between COPD patients and healthy controls based on MB-WCX-MALDI-TOF-MS analysis.

关 键 词：液体芯片-飞行时间质谱 COPD患者 血清蛋白组 疾病标志物 

分 类 号：R730.43[医药卫生—肿瘤]