一株茶树内生淡紫拟青霉的鉴定及抗真菌活性研究  

Identification of An Endophytic Paecilomyces lilaeimls from A Leaf of Camellia sinensis As Well As Determination of Its Anti-fungal Activity

作　　者：李绍锋[1] 杨民和[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《生物灾害科学》2012年第1期45-49,共5页Biological Disaster Science

摘　　要：采用形态学观察及ITS序列分析对1株分离自健康茶树叶片的内生真菌进行鉴定,初步确定该菌株为淡紫拟青霉;并通过对峙培养和发酵液的抑菌试验研究其对真菌的抗生作用。结果表明,淡紫拟青霉FHM-1对7种茶树内生真菌和10种植物病原菌均有较强的抑菌作用;其发酵液对供试的16种真菌具有不同程度的抑制作用,而对拟盘多毛孢的生长有较强的促进作用。In this study,an endophytic fungus from a leaf of Camellia sinensis was identified by morphology and ITS sequence analysis as Paecilomyces lilaeimls.On this basis,confrontation culture and antimicrobial effects of fermentation broth were conducted.Results showed that Paecilomyces lilaeimls FHM-1 had different antimicrobial activities against 7 strains of endophytic fungi and 10 strains of plant pathogenic fungi.The results also showed that fermentation broth of the strain HMF-1 could inhibit 16 kinds of sensitive fungi to different degree,but promoted the growth of Pestalotiopsis sp.

关 键 词：内生真菌 its鉴定 对峙培养 抗真菌作用 

分 类 号：S435.71[农业科学—农业昆虫与害虫防治]