蚓激酶提取条件的优化与酶活性测定  

Optimization of Lumbrokinase Extraction Conditions and Determination of Its Activity

作　　者：陈欲云[1] 杨跃寰[1] 刘春丽[1] 谢万如[1] 

机构地区：[1]四川理工学院化学与制药工程学院,四川自贡643000

出　　处：《四川理工学院学报（自然科学版）》2011年第6期643-645,共3页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

摘　　要：蚯蚓纤溶酶是目前较为理想的溶栓药物之一,具有广泛的临床应用前景。采用盐析法从蚯蚓中提取蚓激酶,通过采用考马斯亮蓝G-250染色法间接地测定蚓激酶活性,优化出最佳的提取条件。结果显示,蚓激酶的提取最佳PH为7.5-8.0,二次盐析硫酸铵饱和度应在80%以上。Lumbrokinase is considered one of the preferred thrombolytic drugs and widely used in clinical.Lumbrokinase is extracted from the earthworms by salt fractionation and lumbrokinase activity is indirectly measured by Coomassie Brilliant Blue G-250 staining.The optimal extraction conditions of the lumbrokinase from the earthworms is determined: the bast pH value is 7.5～8.0 and saturation of ammonium sulfate should be above 80% in the second salting-out.

关 键 词：蚓激酶 提取条件 酶活性 考马斯亮蓝G-250染色法 

分 类 号：Q814[生物学—生物工程]