高效液相色谱法测定豆芽中2,4-二氯苯氧乙酸残留  

Determination of 2,4-dichlorophenoxyacetic acid residues in bean sprout samples by high performance liquid chromatography

作　　者：夏慧[1] 陆自强[2] 杨益众[2] 

机构地区：[1]江苏省泰州出入境检验检疫局,江苏泰州225300 [2]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2011年第4期86-89,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省出入境检验检疫局科研项目(2009KJ32);扬州市科技攻关项目(YZ2010079)

摘　　要：为建立高效液相色谱法测定豆芽中2,4-二氯苯氧乙酸残留量的方法,样品采用碱化水提取,离心、过滤后将提取液酸化,经LC-18固相萃取小柱净化,甲醇洗脱,以甲醇-水(体积比4∶1)为流动相,流速为0.8mL.min-1,检测波长为290nm。用Agilent TC-C18色谱柱分离,PDA紫外检测器测定豆芽中2,4-二氯苯氧乙酸含量。结果表明:该方法2,4-二氯苯氧乙酸的检测限(S/N=3)为0.01mg.kg-1,样品回收率为91.16%～108.35%,RSD为3.51%～6.27%。该方法操作过程简单,灵敏度和准确度较高,重复性好,可用于豆芽中2,4-二氯苯氧乙酸残留量的检测。A method for determination of 2,4-dichlorophenoxyacetic acid residues in bean sprout samples by solid phase extraction-high performance liquid chromatography was developed.2,4-dichlorophenoxyacetic acid was extracted with alkaline water from bean sprout samples and then the total extract was cleaned up by the LC-18 cartridges and eluted by CH3OH after centrifugation,filtration and acidification.CH3OH-H2O(V/V,4∶1) was applied as mobile phase,the flow rate was 0.8 mL·min-1,detection wavelength was 290 nm and the analysis was carried out by separation with Agilent TC-C18 column and determination with PDA detector.Under the experimental conditions the results of determination were as follows: the detection limit of 2,4-dichlorophenoxyacetic acid was 0.01 mg·kg-1(S/N=3),the average fortified recoveries of 2,4-dichlorophenoxyacetic acid in the blank was 91.16%-108.35% and the relative standard deviations(RSD,n=5) was 3.51%-6.27%.The method was simple,sensitive,accurate and repeatable and can be used to determine 2,4-dichlorophenoxyacetic acid residues in bean sprout samples.

关 键 词：豆芽 2 4-二氯苯氧乙酸 高效液相色谱 残留 

分 类 号：TS2[轻工技术与工程—食品科学与工程]