老年人肠道炎性息肉与HLA-DQA1-DQB1连锁基因单倍体的关联研究  

Correlation of intestinal inflammatory polyps with HLA-DQA1-DQB1-linked gene haplotype in elderly patients

作　　者：季洪赞[1] 吴晓尉[1] 孙泉[1] 许小兵[1] 张晓华[1] 赵文新[2] 于正洪[2] 冯根宝[3] 汪芳裕[1] 段苏友[1] 

机构地区：[1]南京军区南京总医院消化内科,南京210002 [2]南京军区南京总医院干部病区,南京210002 [3]南京军区南京总医院内科实验室,南京210002

出　　处：《医学研究生学报》2011年第7期717-720,共4页Journal of Medical Postgraduates

基　　金：南京军区总医院科研基金项目(2005019)

摘　　要：目的人类白细胞抗原(human leucocyte antigen,HLA)参与肠道炎性息肉的病理生理过程,通过HLA介导的炎症因子与功能性肠道障碍(functional bowel disorder,FBD)发生机制有关。文中研究老年患者肠道炎性息肉与HLA-DQA1-DQB1连锁基因单倍体的相关性。方法选择68例正常老年人作为对照组,114例老年肠道炎性息肉合并FBD患者作为研究组。按照FBD在功能性胃肠病罗马Ⅲ标准中分型,所有肠道炎性息肉患者按序数分成4个亚组,包括肠易激综合征(n=30)、功能性腹胀(n=28)、功能性便秘(n=28)、功能性腹泻(n=28)。采用序列特异性引物聚合酶链反应(PCR sequence-spe-cific primer,PCR-SSP)技术,检测各组患者循环血HLA-DQA1-DQB1连锁基因单倍体的频率,并进行各组比较分析。结果研究组各HLA-DQA1-DQB1连锁基因单倍体的检出率与对照组比较,无显著性差异(P>0.05,RR<4),但研究组功能性腹泻亚组DQA1*0301-DQB1*0602连锁基因单倍体检出率(3.57%)低于对照组(20.59%),存在显著性差异(P<0.01,RR=0.14)。结论 DQA1*0301-DQB1*0602连锁基因单倍体与老年人肠道炎性息肉合并功能性腹泻有一定关联,HLA-DQA1-DQB1连锁基因单倍体在老年人肠道炎性息肉患者中分布与正常人之间存在差异。Objective Human leucocyte antigen(HLA) is involved in the pathophysiology of intestinal inflammatory polyp(IIP),as well as in the pathogenesis of functional bowel disorders(FBD) via HLA-mediated inflammatory factors.This article is to investigate the association of the HLA-DQA1-DQB1-linked gene haploid with intestinal inflammatory polyps in elderly patients.MethodsSixty-eight normal elderly subjects were enlisted as controls,and 114 elderly patients with IIP complicated by FBD were included in the case group.According to the sequential numbers of Rome Ⅲ criteria of FBD classification,the patients were divided into 4 subgroups: irritable bowel syndrome(n= 30),functional abdominal distension(n=28),functional constipation(n=28),and functional diarrhea(n=28).The haplotype frequencies of the HLA-DQA1-DQB1-linked genes in the circulating blood were detected by PCR with sequence-specific primers(PCR-SSP),and results were compare among different groups.Results Observer Group in all HLA-DQA1-DQB1 linked genes,The detection rate of HLA-DQA1-DQB1-linked gene haploids showed no significant difference in the case group as compared with the controls(P>0.05,RR<4),however,but that of the DQA1 *0301-DQB1* 0602 chain gene haplotype was significantly lower in the functional diarrhea subgroup than in the control(3.57% vs 20.59%,P<0.01,RR=0.14).Conclusion The DQA1 *0301-DQB1* 0602 chain gene haplotype is correlated with IIP complicated by functional diarrhea in elderly patients,and the distribution of the HLA-DQA1-DQB1-linked gene haplotype in elderly IIP patients differs from that of the healthy controls.

关 键 词：老年人 肠道炎性息肉 功能性腹泻 基因 

分 类 号：R735[医药卫生—肿瘤]