重组人SOD工程菌发酵表达条件的优化  

Optimization of Fermentation Conditions of Recombinant Human Superoxide Dismutase Genenic Engineering Bacteria

作　　者：刘建荣[1] 宋苍苍[1] 

机构地区：[1]河北大学生物工程技术研究中心,河北保定071002

出　　处：《中国医药工业杂志》2011年第7期501-503,562,共4页Chinese Journal of Pharmaceuticals

基　　金：河北省教育厅科学研究计划项目(2006308);河北省科技研究与发展指导计划项目(06276455);河北省生物工程重点学科资助项目(2008011)

摘　　要：采用正交试验优化重组人超氧化物歧化酶工程菌的LB培养基,得到培养基最佳配比(g/L):蛋白胨12,酵母膏5,葡萄糖1和氯化钠10。以此为基础优化基因工程菌发酵条件,确定培养基初始pH 6.5～7.5,装液量20%,菌体密度(D_(600))0.55～1.4,42℃诱导表达2～3 h,目的蛋白相对表达量由优化前的25%提高至41%,绝对表达量是优化前的2.4倍。The LB medium of recombinant human superoxide dismutase genenic engineering bacteria was optimized by orthogonal test.The optimal ratios of medium components(g/L) were as follows:peptone 12,yeast extract 5,glucose 1 and sodium chloride 10.The fermentation conditions were optimized in the above optimal medium.The relative expression rate of rhSOD was increased from 25%to 41%,and the absolute expression rate of rhSOD was found 2.4 times as that before optimization when the incubation parameters were adjusted as follows:the initial pH 6.5-7.5, liquid volume in flask 20%,growth density of the strain(D_(600)) 0.55-1.4,and inducing time at 42℃for 2-3 h.

关 键 词：重组人超氧化物歧化酶 基因工程菌 发酵 诱导 表达 

分 类 号：TQ9[化学工程]