丽格海棠细菌性叶斑病病原菌鉴定  

Identification of Bacterial Leaf Spot Disease Caused by Xanthomonas Campestris pv. Begoniae on Rieger Begonia

作　　者：周丽洪[1] 王永吉[1] 韩阳[1] 张丽辉[1] 姬广海[1] 

机构地区：[1]云南农业大学植物保护学院,昆明650201

出　　处：《西南师范大学学报（自然科学版）》2012年第12期56-61,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：农业部公益性行业专项基金资助项目(201203029)

摘　　要：2011年在云南石林丽格海棠上发现一种由细菌侵染引起的新病害,称为丽格海棠细菌性叶斑病.该病主要危害叶片,症状为叶片边缘形成倒V字型坏死和起皱.从病叶上分离到14株细菌菌株,菌株接种于不同丽格海棠品种上,发病症状与田间自然症状一致,采用柯赫氏法则重新分离得到此病的病原细菌.经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和16SrDNA序列分析以及病原菌的寄主范围测定,确定该病原菌为野油菜黄单胞菌秋海棠致病变种Xanthomonas campestris pv.begoniae.这是中国关于Xcb侵染丽格海棠的首次报道.A new bacterial disease from Rieger begonia,which was first found in Shilin of Yunnan Province in 2011,was main harmful for Rieger begonia leaves.Initial symptoms are small circular or polygonal water-socked spot on leaf margin;later these spots expand and join together,forming bigger inverted V-shaped necrotic specks.14 strains are isolated from diseased leaves from Rieger begonia.Healthy leaves and the same strains have been re-isolated from inoculated leaves by the Koch's rule.

关 键 词：丽格海棠 野油菜黄单胞菌秋海棠致病变种 病原菌鉴定 BIOLOG 16s rDNA 

分 类 号：S685.99[农业科学—观赏园艺]