响应面法优化微生物降解南瓜渣为可溶性多糖  

Application of Box-Behnken Design for Optimizing Microorganism Degradation of Pumpkin Residue to Produce Polysaccharides by Trichoderma Reesei RUT-C30 and White rot fungi 5.0776

作　　者：杨瑞[1] 李全宏[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,农业部果蔬加工重点开放实验室,果蔬加工教育部工程研究中心,北京100083

出　　处：《食品研究与开发》2012年第6期155-158,共4页Food Research and Development

基　　金：国家自然基金(30671469)

摘　　要：南瓜水提可溶性多糖后产生大量不溶性残渣。以提取可溶性多糖后残留的南瓜渣为底物,采用易降解纤维材料的混合菌种(里氏木霉RUT-C30和白腐真菌5.077 6)深层液体发酵降解南瓜渣的方法制备可溶性多糖。利用Design Expert软件响应面法中心组合设计(Box-Benhnken)优化初始pH(X1),白腐真菌延迟接种时间(X2),接种量(X3)对可溶性多糖得率的影响。结果表明,初始pH4.79,白腐真菌延迟接种时间37.44 h,接种量3.11%时可溶性多糖产率最高。在此条件下进行发酵验证,所得多糖得率预测值为48.633 7%,实际值为47.789 4%,相对误差仅为0.844 3%,实验值与模型预测值吻合度较好。Large amounts of insoluble residues were produced after water extraction of soluble polysaccharides from pumpkin.Box-Behnken experimental design was applied to study the effect of initial pH(X1),inoculation time delay of white rot fungus(X2) and inoculums(X3) on the production of soluble polysaccharides from pumpkin residue by Trichoderma reesei RUT-C30 and White rot fungi 5.077 6.The conditions of response surface optimization were: initial pH value of 4.79,inoculation time delay of white rot fungus 37.44 h and inoculums 3.11 %.Under these conditions,the yield of polysaccharides could be achieved to 47.789 4 %,compared to predicted value 48.663 7 %.The result showed that experimental value was fit to model prediction,having high inosculating degree and the error was relatively small.

关 键 词：南瓜 多糖 响应面优化 微生物 

分 类 号：TS201.2[轻工技术与工程—食品科学]