4株鸭疫里默氏杆菌的PCR快速鉴定及药敏试验  

PCR Rapid Identification and Drug Sensitive Test of Four Isolated Strains of Riemerella anatipestifer

作　　者：张蓉蓉[1] 罗青平[1] 温国元[1] 王红琳[1] 杨峻[1] 艾地云[1] 罗玲[1] 邵华斌[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2012年第22期5193-5196,共4页Hubei Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201003012);湖北省农业科技创新中心项目(2011-620-001-003);动物胚胎工程及分子育种湖北省重点实验室开放课题项目(2010ZD150)

摘　　要：从湖北省武汉郊区和监利市两地的发病雏鸭分离纯化到4株鸭疫里默氏杆菌(Riemerella a-natipestifer,RA),通过对该菌形态学鉴定、生化试验、细菌16S rRNA的快速鉴别诊断证明了4株细菌均为鸭疫里默氏杆菌。将不同来源的菌株各选一株HBSPX和HBJL,进行了16S rRNA目的片段的扩增并测序,测序结果表明这两株细菌与其他鸭疫里默氏杆菌的16S rRNA同源性高达99.4%和98.9%。药敏试验结果表明,HBSPX株对阿莫西林、先锋VI高度敏感,但HBJL株对氯洁霉素和红霉素高度敏感。Four strains isolated from diseased duck in Wuhan suburbs and Jianli of Hubei province were identified as Riemerella anatipestifer(RA) by morphological identification,biochemical tests and PCR identification of 16 S rRNA of bacteria.16 S rRNA gene of bacteria was PCR amplified from two isolated strains HBSPX and HBJL.The nucleotide sequence identity of 16 S rRNA between two isolated strains and other RA was 99.4% and 98.9%.In drug sensitive test,HBSPX was highly sensitive to amoxicillin and cephalexin;and HBJL was highly sensitive to clindamycin and erythromycin.

关 键 词：鸭疫里默氏杆菌(Riemerella anatipestifer RA) 16S RRNA 分离鉴定 药敏试验 

分 类 号：S852.61[农业科学—基础兽医学]