三组分大孔树脂的合成及其在脂肪酶固定中的应用  

Synthesis of Macroporous Terpolymers and Its Application in Immobilization of Lipase

作　　者：段洪东[1] 刘文涛[1] 孟霞[1] 王兴建[1] 张娟[1] 

机构地区：[1]山东轻工业学院化学与制药工程学院,山东济南250353

出　　处：《山东科技大学学报（自然科学版）》2012年第3期90-94,共5页Journal of Shandong University of Science and Technology(Natural Science)

基　　金：山东省自然科学基金项目(ZR2011BM011)

摘　　要：以甲苯和正庚烷为致孔剂,采用悬浮聚合法合成了三组分甲基丙烯酸缩水甘油酯-二甲基丙烯酸乙二醇酯-丙烯酸丁酯(GMA-EGDMA-BA)环氧基大孔树脂,并应用于脂肪酶的固定。结果表明,加入第三单体丙烯酸丁酯后,同GMA-EGDMA相比较,GMA-EGDMA-BA树脂的孔径变大,其固定化酶量提高约1倍(达20mg/g);同游离酶相比较,固定化酶对温度和pH值的适应性有所提高,其活性恢复达到83.3%以上。The macroporous terpolymers with epoxy groups including the glycidyl methacrylate(GMA),ethylene glycol dimethacrylate(EGDMA) and butyl methacrylate(BA) were synthesized by suspension polymerization method,taking the toluene and n-heptane as porogen,and meanwhile,the terpolymers were used for immobilization of lipase.The results showed that after adding the third monomer butyl methacrylate,the lipase binding capacity of GMA-EGDMA-BA was 20 mg protein/g(polymer),twice as much compared with GMA-EGDMA and the pore size of poly(GMA-EGDMA-BA) was enlarged.The immobilization of lipase gave more than 83.3% of active recovery and showed a broader pH and higher temperature stability.

关 键 词：三组分树脂 固定化脂肪酶 温度 活性恢复 

分 类 号：O632.52[理学—高分子化学]