幽门螺杆菌硫氧还蛋白1表达量对胃癌BCG-823细胞生长的影响  

The roles of helicobacter pylori with different expressed thioredoxin-1 on the growth of gastric cancer cell BCG-823

作　　者：张月霞 刘琳娜[1] 丁士刚[1] 石岩岩[1] 张婷[3] 张静[1] 王晔[1] 蓝宇[2] 

机构地区：[1]北京大学第三医院消化科,100191 [2]北京积水潭医院消化内科 [3]北京大学医学部病原生物学系

出　　处：《中华临床医师杂志（电子版）》2012年第16期4598-4602,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(30770980)

摘　　要：目的研究幽门螺杆菌(Hp)不同表达量硫氧还蛋白1(Trx1)对人胃癌细胞(BCG-823)生长的作用。方法将高表达Trx1及低表达Trx1Hp分别以1∶50,1∶100,1∶200细胞细菌浓度比进行细菌细胞共培养,设不加菌空白对照组,分别于24h及48h后分别用倒置显微镜及MTT法观察细胞形态及细胞存活率,并收集1∶100浓度组细胞用流式细胞仪检测细胞周期。结果从镜下观察及测细胞OD值均发现Hp可使BCG-823细胞增生活跃,并呈浓度及时间依赖性,高Trx1组细胞存活率明显高于对应时间及浓度的低Trx1组,两组比较有统计学差异;用流式细胞仪检测细胞周期结果表明高Trx1组BCG-823细胞进入S期的比例于24h及48h均明显高于低Trx1细菌组,且无亚二倍体出现,结果有统计学差异。结论高表达Trx1的Hp对胃癌细胞有促增殖作用,Trx1与Hp的致癌机制可能有关。Objective To investigate the effect of Helicobacter pylori(Hp)with different expressed thioredoxin-1(Trx1)on the growth of gastric cancer cell BCG-823.Methods There were three groups:High-expressed Trx1 Hp group,low-expressed Trx1 Hp group and the control group without Hp.BCG-823 cells were co-cultured with different proportions of Hp(1∶50,1∶100,1∶200)for 24 and 48 hours respectively.Morphology changes of BCG-823 cells were observed by inverted microscope.The cell proliferation was assessed by MTT spectrophotometric analysis.The cell cycles(1∶100 group)were measured by flow cytometry(FCM).Results The results of inverted microscope and MTT assay showed that Hp could promote cell growth of BCG-823,which was concentration-dependent and time-dependent.The cell survival rate of high-expressed Trx1 group was significantly higher than the group of low-expressed Trx1,and there were significant differences between the two groups(P<0.05).The result of FCM showed that the percentage of the cells in S phase with high-expressed Trx1 group was significantly increased than the low-expressed Trx1 group at both 24 h and 48 h(P<0.05),and hypodiploids were not found.Conclusions Hp with high-expressed Trx1 may promote the proliferation of gastric cancer cells BCG-823,and has potential function in tumor genesis.

关 键 词：螺杆菌 幽门 硫氧还蛋白质类 胃肿瘤 细胞周期 BCG-823 

分 类 号：R735.2[医药卫生—肿瘤]