七氟醚后处理对大鼠脑缺血再灌注后兴奋性氨基酸的影响  

Effect of sevoflurane post-treatment on excitatory amino acid after cerebral ischemia and reperfusion

作　　者：陈红生[1] 范冰冰[2] 张红 许忠玲[1] 

机构地区：[1]南通大学附属医院麻醉科,江苏省226001 [2]南通大学医学院 [3]南京医科大学附属鼓楼临床学院

出　　处：《中华临床医师杂志（电子版）》2012年第21期6772-6774,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：南通大学医学院科研基金(2011)

摘　　要：目的观察七氟醚后处理对大鼠局灶性脑缺血再灌注损伤脑组织中兴奋性氨基酸的影响。方法雄性大鼠90只,随机分为假手术组(C组)、脑缺血再灌注组(I/R组)、七氟醚后处理组(S组),建立大鼠大脑中动脉阻断再灌注模型(MCAO/R),脑缺血再灌注后24 h、48 h和72 h时观察脑缺血再灌注后神经行为学评分,同时检测脑组织内兴奋性氨基酸谷氨酸(Glu)和天门冬氨酸(Asp)的含量。结果与C组比较,I/R组和S组大鼠神经行为学评分增加(P<0.01),S组Glu和Asp的浓度上升(P<0.05),I/R组Glu和Asp的浓度上升明显(P<0.01)。S组大鼠行为学评分低于I/R组(P<0.01),脑组织中Glu和Asp浓度较脑I/R组低(P<0.05)。结论七氟醚后处理减轻大鼠脑缺血再灌注损伤,其作用机制可能通过降低脑组织中Glu和Asp含量,达到神经功能保护作用。Objective To observe the sevoflurane treatment on focal cerebral ischemia and reperfusion injury in brain tissue excitatory amino acid.Methods 90 male rats were randomly divided into sham operation group(group C),cerebral ischemia and reperfusion group(I/R group)and sevoflurane post-processing group(group S).The middle cerebralartery occlusion reperfusion model(MCAO/R) was established,neurobehavioral scores after cerebral ischemia and re-perfusion 24 h,48 h and 72 h were observed,the content of the excitatory amino acid of brain tissue were detected simultaneously.Results Compared with group C,neural behavior scores increased in the I/R group and group S(P<0.01).Besides concentration of excitatory amino acid increased significantly in I/R group(P<0.01);concentration of excitatory amino acid increased significantly in group S(P<0.05).The be-havioral score of evoflurane treated rats belowed the cerebral ischemia and reperfusion group(P<0.01),concentrations of excitatory amino acid were significantly lower than cerebral ischemia and reperfusion group(P<0.05).Conclusions Sevoflurane postprofessing can decrease the ischemia and reperfusion injury,its mechanism may protect nerve function by increasing content of excitatory amino acid.

关 键 词：再灌注损伤 兴奋性氨基酸 麻醉药 吸入 后处理 

分 类 号：R743.3[医药卫生—神经病学与精神病学]