雄激素非依赖性前列腺癌LNCaP-AI细胞亚系模型的建立  

Establishment of androgen-independent human prostate cancer cell line model LNCaP-AI

作　　者：孔德玉[1] 杨冬梅[1] 陈艳媛[1] 王宇[1] 张金桥[1] 高萌[1] 方芳[1] 

机构地区：[1]吉林医药学院,吉林吉林132013

出　　处：《吉林医药学院学报》2012年第6期361-363,共3页Journal of Jilin Medical University

基　　金：吉林医药学院大学生科研基金资助项目(2011-13)

摘　　要：目的利用雄激素递减法建立雄激素非依赖性前列腺癌(AIPC)细胞亚系模型LNCaP-AI。方法利用活性炭/葡聚糖处理的血清模拟去雄激素环境培养LNCaP细胞,逐渐递减培养基中雄激素10 d,然后在无雄激素环境中继续培养6个月,培养出能够在无雄激素环境中生长的LNCaP-AI细胞亚系。采用MTT和ELISA法检测LNCaP-AI细胞在去雄激素环境下的增殖能力和前列腺特异性抗原(PSA)的水平。结果 LNCaP细胞在去雄激素环境培养初期生长缓慢,细胞形态呈神经内分泌样改变,经过6个月的连续无雄激素培养,细胞恢复以前的形态和生长。MTT检测表明LNCaP-AI细胞能够在去雄激素环境中生长,通过ELISA检测PSA的分泌提示LNCaP-AI细胞能够在去雄激素环境中恢复分泌PSA的功能。结论激素递减法成功的建立了AIPC细胞亚系模型LNCaP-AI,利用该模型能够模拟前列腺癌由雄激素依赖发展为雄激素非依赖的过程。Objective To establish androgen-independent human prostate cancer cell line LNCaP-AI by gradual deprivation of hormone.Methods LNCaP cells were cultured in the medium with gradual deprivation of hormone(treated by active carbon/dextran to simulate androgen deprivation) for 10 days;and then the cells were cultured with complete deprivation of androgen for 6 months till the cell could live without androgen to established cell line LNCaP-AI.The growth and secretion of PSA of LNCaP-AI cell in absence of hormone were examined by MTT and ELISA.Results LNCaP cells grew slowly after deprivation of androgen and took on a neuroendocrine phenotype.After 6 months' non-androgen culture,the cells regained original morphology and growth.LNCaP-AI cells could grow in non-androgen condition by MTT assay.ELISA assay indicated that LNCaP-AI cells could regain PSA-secreting activity in non-androgen condition.Conclusion Androgen-independent LNCaP cell line can be established by culturing with gradual deprivation of hormone and is an ideal model which mimic the process from androgen-dependent to androgen-independent in prostate cancer cell line.

关 键 词：前列腺癌 雄激素非依赖 LNCAP 

分 类 号：R737.25[医药卫生—肿瘤]