大鼠下肢缺血性梗死模型的制备  

Preparation of lower extremity ischemic infarct model in rats

作　　者：吕桂阳[1] 刘志红[1] 朱玉萍[1] 张雪[1] 吴传洋 赵东海[2] 

机构地区：[1]吉林医药学院临床医学院,吉林吉林132013 [2]吉林医药学院病理教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2012年第6期383-385,共3页Journal of Jilin Medical University

基　　金：吉林医药学院大学生科研基金课题(201001)

摘　　要：目的以不同方法制备大鼠下肢缺血性梗死模型,比较不同制备方法间的优劣,建立有效的下肢缺血性梗死模型。方法 32只Wistar大鼠随机分为对照组、直接结扎组、股浅动脉结扎组和股动脉结扎组4组,每组8只。对照组切开皮肤,不做结扎,作为创伤对照模型。直接结扎组用手术线直接结扎后肢。股浅动脉结扎组和股动脉结扎组分别分离股浅动脉、股动脉,并用手术线结扎血管。观察下肢皮肤和肌肉变化,术后1周处死大鼠取下肢肌肉组织进行HE染色。结果与对照组和股浅动脉结扎组相比,直接结扎法与股动脉结扎法均可使大鼠后肢出现明显的缺血坏死,但直接结扎法造成损伤面积过大并有严重的炎症反应,股动脉结扎法可造成下肢明显缺血坏死且炎症反应轻微。结论股动脉结扎法是制备大鼠下肢缺血性梗死模型的有效方法。Objective To prepare the lower extremity ischemic infarct model in rats by different ways and to explore the advantages and disadvantages of different methods to construct effective lower extremity ischemic infarct model.Methods Thirty two rats were randomly divided into four groups with eight rats in each group:control group,direct ligation group,superficial femoral artery ligation group and femoral artery ligation group.No artery was ligated in control group only to dissect skin.Hindlimb was ligated by stitches in direct ligation group.Superficial femoral artery and femoral artery was ligated respectively in superficial femoral artery ligation group and femoral artery ligation group.The change of skin and muscle in lower extremity was observed and HE staining was undertaken after one week of operation.Results Compared with the control group and the superficial femoral artery ligation group,Ischemic necrosis was significantly happened in the direct ligation group and the femoral artery ligation group.The injury area was just major and inflammation reaction was serious in the direct ligation group.The inflammation reaction was slight and lower limb ischemia was significant in the femoral artery ligation group.Conclusion Femoral artery ligation is a modus operandi to prepare lower extremity ischemic infarct model.

关 键 词：大鼠 下肢缺血性梗死 模型 

分 类 号：R364.2[医药卫生—病理学]