透明颤菌vgb基因在产核黄素B.subtilis中的整合表达研究  

Integrated Expression of vitreoscilla hemoglobin gene(vgb) in Bacillus subtilis for riboflavin Production

作　　者：张西锋[1] 王丽梅[1] 李万芬[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]武汉星辰现代生物工程有限公司,湖北武汉430074

出　　处：《生物技术》2012年第2期26-29,共4页Biotechnology

基　　金：武汉工业学院引进(培养)人才科研启动资金资助项目(2010RZ16)资助

摘　　要：目的:将透明颤菌血红蛋白vgb基因应用于核黄素的工业化生产。方法:以枯草芽孢杆菌整合载体pAmyE构建了vgb基因的整合表达载体pAudV,采用化学转化法将vgb基因整合到枯草芽孢杆菌GJ08的染色体上,并通过发酵摇瓶实验检测核黄素的产量。结果:得到产核黄素枯草芽孢杆菌GJ09,摇瓶试验结果表明,在限氧条件下核黄素的产量分别提高了5.23%和3.42%。结论:透明颤菌血红蛋白vgb基因能够促进核黄素产量的提高,可以应用于核黄素的工业化生产中。Objective:Research the role of Vitroscilla Hemoglobin gene(vgb) in B.subtilis and apply with the industrial production of riboflavin.Method:Integrated expression vector pAudV was constructed using plasmid pAmyE.Using double crossover homologous recombination between plasmid pAudV and B.subtilis GJ08,vgb gene was integrated into chromosome of B.subtilis GJ08.Result:Got engineering B.subtilis GJ09,and the results of shake flask shown that:the production of riboflavin of B.subtilis GJ09 in middle and low aeration fermentation were increased by 5.23% and 3.42% than that of B.subtilis GJ08.These results indicated that the production of VHb could improve the production of riboflavin.Conclusion:vitreoscilla hemoglobin gene(vgb) gene could be applied to industrial production of riboflavin.

关 键 词：透明颤菌 VGB基因 核黄素 枯草芽孢杆菌 

分 类 号：Q786[生物学—分子生物学]