一株阿尔泰虫草内生菌的分离及抑菌活性  

Segregation and Bacteriostasis Research of Endophytes from Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora

作　　者：马赟[1] 索菲娅[1] 王傲立[1] 黄罗冬[1] 叶星[1] 张琳[1] 李萍[1] 龚健[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《生物技术》2012年第2期64-67,共4页Biotechnology

基　　金：新疆教育厅项目(XJEDU2010I12);新疆科技厅项目(201091245);自治区绿洲生态重点实验室资助项目(XJDS0206-2010-01)资助

摘　　要：目的:从采自阿尔泰山脉的虫[Ophiocordyceps gracilis(Grev.)G.H.Sung,J.M.Sung,Hywel-Jones&Spatafora]体内分离出1株出现频率较高的真菌(命名为SFY00C3)进行了研究。方法:经形态学和ITS序列分析鉴定,对SFY00C3菌株及其发酵液采用滤纸片法,同时对6种供试菌株不同程度进行了抑制作用的研究。结果:SFY00C3菌株为青霉属(Penicillium)真菌变灰青霉(Penicillium canescens);对大肠杆菌和金黄色葡萄球菌的抑制作用较强,抑菌圈均大于10 mm。结论:虫草内生菌变灰青霉的抑菌活性成分值得进一步研究,为研究新的天然产物抑菌药物提供思路。Objective:A strain of fungal seperated form Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora was highly frequently appeared,and was researched in this experiment.Method:To classify this fungal,morphology evidence and ITS sequencing was applied.Various bacteriostasis ability of this fungal and its broth against 6 strains was tested by the filter paper method too.Result:SFY00C3 is Penicillium canescen,whose broth performed a stronger bacteriostasis to Escherichia coli and Staphylococcus aureus as creating inhibition zone exceed 10mm in diameter.Conclusion:Penicillium canescens,the entophyte of Ophiocordyceps gracilis(Grev.) G.H.Sung,J.M.Sung,Hywel-Jones & Spatafora,is worthwhile further researching to offer new means of developing new natural anti-bacterial drugs.

关 键 词：阿尔泰虫草 内生菌 分离 抑菌活性 

分 类 号：S567.39[农业科学—中草药栽培] S182[农业科学—作物学]