内皮屏障抗原在实验性大鼠脑出血后的表达及其与血脑屏障通透性改变的关系  

The expression of endothelial barrier antigen after intracerebral hemorrhage in rat and its influence to permeability of BBB

作　　者：王晓宇[1,2] 薛维爽[3] 李明[4] 滕伟禹[3] 

机构地区：[1]中国医科大学 [2]辽阳中心医院神经内科,辽宁辽阳111000 [3]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [4]中国医科大学附属第四医院神经内科,辽宁沈阳110032

出　　处：《中风与神经疾病杂志》2012年第2期119-121,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨内皮屏障抗原(EBA)在脑出血(ICH)后血肿周围脑组织中的表达及其在ICH后血脑屏障(BBB)通透性改变中的作用。方法利用立体定向技术向大鼠尾状核内注入50μl自体股动脉血建立ICH模型,用免疫组化法检测ICH后血肿周围EBA、纤维蛋白原的蛋白表达、Belayev法测定BBB通透性及干湿重法测定脑水含量的动态变化。结果 ICH后6hEBA表达开始减少,3d时最少,7d略有回升;纤维蛋白原表达、BBB通透性、脑水含量均于ICH后6h开始升高,3d达高峰,7d逐渐下降,ICH后EBA蛋白表达与脑水含量呈负相关(r=-0.840,P<0.01),与EB含量负相关(r=-0.827,P<0.01),与纤维蛋白原表达呈负相关(r=-0.851,P<0.01)。结论大鼠实验性ICH后EBA的表达减少,且与纤维蛋白原、BBB通透性及脑水含量增高具有明显的相关性。Objective To investigate the expression of endothelial barrier antigen(EBA) after intracerebral hemorrhage(ICH),and the impact on the permeability of the blood-brain barrier(BBB).Methods 50μl of autogenetic blood from femoral artery was injected into caudate nucleus of mouses by stereotaxy technique.The level of EBA expression and fibrinogen was measured by immunohistochemistry,permeability of BBB was assessed by Belayev's method and the brain water content was determined by the method of wet-dry weight.Results The expression of the fibrinogen,Evans blue(EB) of the brain tissue and the brain water content started to increase at 6h after ICH,reached the peak on 3d,and gradually decreased after 7 days,while the opposite trend of the expression of EBA was observed.Conclusion The expression of EBA after operated ICH decreased,which has a significant correlation with fibrinogen,the permeability of BBB and the brain water content.

关 键 词：脑出血 内皮屏障抗原 纤维蛋白原 脑水含量 血脑屏障 

分 类 号：R743.34[医药卫生—神经病学与精神病学]