芦丁的酶法转化及其产物异槲皮苷的分离  

The enzymatic transformation of rutin and the separation of the hydrolysate isoquercitrin

作　　者：吴迪[1] 刘廷强[1] 王东明[1] 金凤燮[1] 鱼红闪[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2012年第6期399-401,共3页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅创新团队项目(2009T007)

摘　　要：利用微生物Absidiasp.R9g产的芦丁-α-鼠李糖苷酶(RhaR)转化芦丁制备异槲皮苷,采用硅胶柱层析法对酶解产物进行分离纯化。结果表明,24.0g芦丁的酶解产物,经分离可得到较纯的异槲皮苷单体13.1g,其得率为49.4%。通过高效液相色谱检测其纯度达到98.3%。Isoquercitrin was transformation from rutin by rutin-α-rhamnosidase(RhaR) produced with Absidiasp.R9g.Hydrolysate was separated from 24.0g rutin by silica gel column and 13.1g isoquercitrin was obtained.The yield was 49.4%and the purity of the product was 98.3%.

关 键 词：芦丁-α-鼠李糖苷酶 酶转化 异槲皮苷 

分 类 号：R284[医药卫生—中药学]