激素抵抗性哮喘患者外周血CD4^+CD25^+Foxp3^+调节性T细胞及IL-10、TGF-β_1的变化及意义  

Changes and clinical significance of CD4^+CD25^+Foxp3^+ regulatory T cell and IL-10 , TGF-β_1 in steroidresistant asthma patients of peripheral blood

作　　者：钱一龙[1] 赵振中[1] 朱建俊[1] 谢中华[1] 王珠美[1] 

机构地区：[1]杭州市余杭区第二人民医院内二科,311121

出　　处：《中华哮喘杂志（电子版）》2013年第2期95-98,共4页Chinese Journal of Asthma(Electronic Version)

摘　　要：目的观察激素抵抗性哮喘(SRA)患者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)及白介素10(IL-10)、转化生长因子β1(TGF-β1)的变化,分析其在SRA发病机制中的作用。方法采用流式细胞术检测40例SRA患者(激素抵抗组)外周血单个核细胞CD4+CD25+Foxp3+Treg数目,并计算CD4+CD25+Foxp3+Treg占CD4+T淋巴细胞的百分比;酶联免疫吸附试验(ELISA)法检测其血清IL-10、TGF-β1水平,并与激素敏感性患者(激素敏感组,46例)及正常体检者(正常组,30例)进行对比。结果激素抵抗组患者外周血CD4+CD25+Foxp3+Treg占CD4+T淋巴细胞的百分比、CD4+CD25+Foxp3+Treg绝对值及血清IL-10、TGF-β1水平均明显低于激素敏感组与正常组(P<0.01,P<0.05);激素敏感组患者外周血CD4+CD25+Foxp3+Treg占CD4+T淋巴细胞的百分比、CD4+CD25+Foxp3+Treg绝对值及血清TGF-β1水平明显低于正常组(P<0.01,P<0.05),血清IL-10无明显差异(P>0.05);CD4+CD25+Foxp3+Treg/CD4+T及CD4+CD25+Foxp3+Treg绝对数均与血清IL-10、TGF-β1水平呈明显正相关(P<0.01)。结论 SRA患者外周血CD4+CD25+Foxp3+Treg数目减少及IL-10、TGF-β1含量减低可能与的发生、发展有关。Objective To investigate the changes of CD4+CD25+Foxp3+ regulatory T cell and IL-10 , TGF-β1 in steroid-resistant asthma patients of peripheral blood , to study their significance. Methods The CD4+CD25+Foxp3+ regulatory T cell in 40cases steroid-resistant asthma patients were detected by flow cytometry.The levels of serum IL-10and TGF-β1 were detected by ELISA.The control groups were 30individuals having general physical examination and 46patients with steroid-sensitive patients.Results The proportion and number of CD4+CD25+Foxp3+ regulatory T cell and the levels of serum IL-10 , TGF-β1 in steroid-resistant asthma group of Peripheral Blood were decreased significantly than steroid-sensitive group and healthy control group ( P <0.01 , P <0.05 ) .The proportion and number of CD4+CD25+Foxp3+ regulatory T cell and the levels of serum TGF-β1 in steroid-sensitive group were decreased significantly than healthy control group , but the levels of serum IL-10 were no statistical difference between them.The proportion and number of CD4+CD25+Foxp3+ regulatory T cell were positively correlated with the serum IL-10 , TGF-β1 ( P <0.01 ) .Conclusions Lower proportion and number of CD4+CD25+Foxp3+ regulatory T cell and lower IL-10 , TGF-β1 were observed in steroid-resistant asthma patients , which might play an important role in occurrence and development of steroid-resistant asthma.

关 键 词：激素抵抗性哮喘 调节性T细胞 转化生长因子Β1 白介素-10 

分 类 号：R73[医药卫生—肿瘤] R56[医药卫生—临床医学]