肠易激综合征患者IL-8和TNF-α含量变化的意义  

The Role of IL-8 and TNF-α in the Pathogenesis of Irritable Bowel Syndrome

作　　者：张茹[1] 王福贤[1] 

机构地区：[1]山东省菏泽市立医院,菏泽274031

出　　处：《临床消化病杂志》2004年第4期167-168,共2页Chinese Journal of Clinical Gastroenterology

摘　　要：目的:本研究通过检测肠易激综合征(IBS)患者血清中FNF-α和IL-8的含量,旨在通过观察它们在IBS患者中的变化来窥探IBS发病机制中有无炎症免疫因素参与,从而进一步探讨能否从改善机体免疫力的角度来治疗IBS。方法:应用酶联免疫吸附试验(ELISA)方法(ABC法)检测了32例腹泻型IBS及18例便秘型IBS患者血清中的TNF-α和IL-8的含量。结果:(1)正常对照组、便秘型IBS患者血清内可测不到TNF-α,腹泻型IBS患者血清内测到低浓度的TNF-α。(2)腹泻型IBS患者血清IL-8含量显著高于正常(P<0.05),便秘型IBS患者血清内IL-8水平略高于正常对照组,但差异无显著性(P>0.05)。(3)相关分析表明,腹泻型IBS患者血清TNF-α与IL-8水平呈正相关(r=0.928,P<0.01)。结论:(1)腹泻IBS患者血清内TNF-α和IL-8含量均较正常升高,提示腹泻型IBS有炎症因子活化。(2)例秘型IBS患者TNF-α、IL-8与正常无显著差异,提示便秘型IBS和腹泻型IBS的免疫紊乱有着不同的病理生理机制。(3)腹泻型IBS患者IL-8的分泌伴随着TNF-α的增多,TNr-α可能通过活化NF-kB来刺激IL-8的分泌。Objective:The aim of this study is to evaluate the role of immunological factors on IBS through observing the changes of IL-8 and TNF-α in patients was IBS, and further discuss whether we can treat IBS through improving immunity. Methods: A total of SO patients was IBS were diagnosed by using the Rome Ⅱ criteria.25 healthy individuals participated in the study. The enzyme linked immunosorbent assay (ARC assay) was used to detect the level of IL-8 and TNF-α in patients with IBS and in normal controls. Results: (1) TNF-α is undectable in normal controls and patients with constipation-predominant IBS,while is elevated in patients with diarrhea-predominant IBS. (2)IL-8 is significantly increased in diarrhea-predominant IBS than in normal controls( P < 0.05) .while there is no difference between patients with constipation-predominant IBS and normal controls(p > 0.05). (3)The correlation coefficients between TNF-α leave and IL-8 level is positive in patients with diarrhea-predominant IBS. Conclusion: (1)The concentration of TNF-a and IL-8 are all higher in diarrhea-predominant IBS than in normal controls, which indicates that inflammation factors take part in the etiology of diarrhea-predominant IBS. (2)There are no difference between TNF-a, IL-8 in constipation predominant IBS and normal controls. That will lead to deregulation of cellular immune function. (3)It is possible that TNF-a activates the transcription of IL-8 gene through NF-kB pathway in diarrhea -predominant IBS.

关 键 词：肿瘤坏死因子白细胞介素 肠易缴综合征 发病机制 

分 类 号：R574[医药卫生—消化系统]