Quantitative Metabolomics:Analysis on Active Components in Extracts from Kaki Folium  

作　　者：DAI Li-peng GU Yuan YIN Ren-jie LIU Chang-xiao SI Duan-yun 

机构地区：[1]State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China [2]School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China [3]Hutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd., Guangzhou 510515, China

出　　处：《Chinese Herbal Medicines》2012年第4期276-281,共6页中草药（英文版）

基　　金：National Basic Research Plan (973 Plan) Foundation of China (2010CB735602);National Innovation Plan for New Drugs (2011ZX09304-002)

摘　　要：Objective In order to analyze the active components in the extracts from Kaki Folium(KF),quantitative metabolomics approach was adopted to investigate the number of active components existing among the different extracts and their variation.Methods LC-MS method was established for the quantitative determination of the active components taking the mixture with reference substance as tested sample.Results In terms of the number of active components and amount presented in the different tested samples of KF extracted by many types of solvents,variation was observed.But rutin,astragalin,and kaempferol were presented in all samples.Difference was found between the samples extracted from the products on market and from the raw materials of KF processed by polar solvents with different recipes.However,the three active components were found in all samples examined.Conclusion These results might be valuable as all information and could be used for the optimization of raw materials extraction procedure to enhance the productivity.Objective In order to analyze the active components in the extracts from Kaki Folium(KF),quantitative metabolomics approach was adopted to investigate the number of active components existing among the different extracts and their variation.Methods LC-MS method was established for the quantitative determination of the active components taking the mixture with reference substance as tested sample.Results In terms of the number of active components and amount presented in the different tested samples of KF extracted by many types of solvents,variation was observed.But rutin,astragalin,and kaempferol were presented in all samples.Difference was found between the samples extracted from the products on market and from the raw materials of KF processed by polar solvents with different recipes.However,the three active components were found in all samples examined.Conclusion These results might be valuable as all information and could be used for the optimization of raw materials extraction procedure to enhance the productivity.

关 键 词：active components ASTRAGALIN extracts from Kaki Folium KAEMPFEROL metabolomics quantitative metabolomics RUTIN 

分 类 号：R284.1[医药卫生—中药学]