美蓝还原酶法应用于突发水污染微生物指标快速检测的研究  

Study on methylene blue reductive enzymatic application in rapid detection on microorganism for sudden water pollution

作　　者：邱颖[1] 王敏娣[1] 邱贺民[1] 

机构地区：[1]廊坊市疾病预防控制中心,河北廊坊市065000

出　　处：《热带病与寄生虫学》2013年第2期89-90,共2页Journal of Tropical Diseases and Parasitology

基　　金：廊坊市科学技术研究与发展计划项目(2012013063)

摘　　要：目的探讨美蓝还原酶法快速检测突发水污染微生物指标即菌落总数的可行性。方法通过对美蓝的褪色时间与GB/T5750.12-2006平板计数法的比较,对菌落总数进行检测。结果美蓝的还原褪色时间与菌量的多少呈反比,即菌量越大美蓝褪色时间越短。结论美蓝还原酶法操作简便,快速,结果准确可靠,可应用于突发水污染的菌落总数的快速检测。Objective To study the feasibility that methylene blue reductase method to the rapid detection on microorganism index in sudden water pollution.Methods The total number of colonies was tested by the comparison between the fading time of methylene blue and the GB/T5750.12-2006 plate count method.Results There was an inverse relationship between the fading time of methylene blue and total number of colonies.The more the number of colonies,the shorter the fading time of methylene blue.Conclusion Methylene blue reduction method is simple,rapid,accurate,reliable and can be applied to rapid detection of the total bacterial colonies in sudden water pollution.

关 键 词：美蓝还原酶法 菌落总数 快速检测 突发水污染 

分 类 号：R38[医药卫生—医学寄生虫学] X83[医药卫生—基础医学]