Simultaneous Determination of Four Major Steroidal Saponins in seven species of Dioscorea L.By HPLC-ELSD  

机构地区：Zhejiang Chinese Medical University, Hangzhou 310053, China[1] Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China[2]

出　　处：《Chinese Herbal Medicines》2011年第1期-,共5页中草药（英文版）

摘　　要：Objective To control the quality of the species in Dioscorea L.better.Methods An HPLC-ELSD method was developed for the first time to simultaneously determine four bioactive ingredients:dioscin,gracillin,protoneodioscin,and protoneogracillin in 31 samples belonging to seven species of Dioscorea L.from different areas.The column was an Inertsil HILIC(250 mm×4.6 mm,5μm).The separation was carried out with a gradient program.The mobile phase was acetonitrile-water at a flow rate of 0.8 mL/min.Results The standard curve was rectilinear in the range of 0.464-12.97μg(r=0.9969)for dioscin,0.310-7.09μg(r=0.9953)for gracillin,0.469- 11.66μg(r=0.9970)for protoneodioscin,and 0.276-6.87μg(r=0.9992)for protoneogracillin.The recoveries of the markers were 98.1%,100.1%,97.2%,and 96.4%,respectively.The contents of the four components were quite different among the seven species of Dioscorea L.Conclusion The proposed HPLC-ELSD method is convenient,fast,accurate,and applicable for simultaneous analysis of multiple bioactive components of species in Dioscorea L.for quality control,which could facilitate discovering new natural resources of steroidal saponin.Objective To control the quality of the species in Dioscorea L.better.Methods An HPLC-ELSD method was developed for the first time to simultaneously determine four bioactive ingredients:dioscin,gracillin,protoneodioscin,and protoneogracillin in 31 samples belonging to seven species of Dioscorea L.from different areas.The column was an Inertsil HILIC(250 mm×4.6 mm,5μm).The separation was carried out with a gradient program.The mobile phase was acetonitrile-water at a flow rate of 0.8 mL/min.Results The standard curve was rectilinear in the range of 0.464-12.97μg(r=0.9969)for dioscin,0.310-7.09μg(r=0.9953)for gracillin,0.469- 11.66μg(r=0.9970)for protoneodioscin,and 0.276-6.87μg(r=0.9992)for protoneogracillin.The recoveries of the markers were 98.1%,100.1%,97.2%,and 96.4%,respectively.The contents of the four components were quite different among the seven species of Dioscorea L.Conclusion The proposed HPLC-ELSD method is convenient,fast,accurate,and applicable for simultaneous analysis of multiple bioactive components of species in Dioscorea L.for quality control,which could facilitate discovering new natural resources of steroidal saponin.

关 键 词：DIOSCIN Dioscorea L. gracillin HPLC-ELSD protoneodioscin protoneogracillin 

分 类 号：R2[医药卫生—中医学]