经选择的肺腺癌患者中EML4-ALK融合基因发生率的研究  

Study of incidence of EML4-ALK confusion gene in selected lung adenacarcinomas

作　　者：孟丽娟[1] 王峻[1] 樊卫飞[1] 蒲骁麟[1] 王琳[1] 刘福银[1] 杨民[1] 

机构地区：[1]江苏省老年医学研究所血液肿瘤科,江苏省南京市210024

出　　处：《实用老年医学》2013年第10期819-822,共4页Practical Geriatrics

基　　金：江苏省科技厅基础研究计划(自然科学基金)面上研究项目(编号:BK2011842);江苏省省级机关医院院级课题(编号:L200903)

摘　　要：目的探讨表皮生长因子受体(EGFR)野生型肺腺癌患者EML4-ALK融合基因的发生率是否高于EGFR状态未明者。方法经病理确诊的不吸烟或少吸烟的肺腺癌患者100例,EGFR野生型组50例,EGFR状态未明组50例,采用原位荧光杂交法(FISH)检测EML4-ALK融合基因状态。结果EGFR野生型组15例(30%)ML4-ALK融合基因阳性;EGFR状态未明组4例(8%)EML4-ALK融合基因阳性,2组差异有显著性(P<0.05);EML4-ALK融合基因发生率与年龄、性别、临床分期无明显相关性(P>0.05)。结论在不吸烟或少吸烟的肺腺癌患者中,经EGFR基因状态筛选可提高EML4-ALK融合基因检测阳性率。Objective To compare the incidence of EML4-ALK confusion gene in lung adenacarcinomas in epidermal growth factor receptor(EGFR) wild group and in EGFR status unknown group.Methods 100 patients suffering lung adenacarcinomas in non-smoker or seldom smoker were divided into 2 groups according to EGFR status,the EGFR wild group(50 patients),and the EGFR status unknown group(50 patients).EML4-ALK confusion gene was detected by fluorescence in site hybridization(FISH).Results In EGFR wild group,EML4-ALK confusion gene existed in 15 out of 50(30%) patients,while in EGFR status unknown group,only in 4 out of 50(8%) patients.The positive percentage of EML4-ALK confusion gene was significantly higher in the EGFR wild group that than in the EGFR status unknown group(P < 0.05).The incidence of EML4-ALK confusion gene was not associated with age,gender and clinical stage(P > 0.05).Conclusions Selecting patients in non-smoker or seldom smoker by detecting EGFR can improve the positive percentage of EML4-ALK confusion gene.

关 键 词：EML4-ALK融合基因 表皮生长因子受体 肺腺癌 原位荧光杂交法 

分 类 号：R734.2[医药卫生—肿瘤]