发生与未发生抗结核药物诱导肝损伤患者血浆中差异性表达的微RNAs分子筛查研究  

Differential expression of plasma microRNAs in patients with and without anti-tuberculosis drugs-induced hepatotoxicity

作　　者：叶长根 陈彩萍[2] 刘亮明[2] 谢平[2] 朱伟星[2] 白茹[2] 赵晖[2] 吴剑琴[2] 夏利萍[2] 

机构地区：[1]景德镇市第三人民医院消化科 [2]上海交通大学附属第一人民医院松江分院感染科,201600

出　　处：《中华临床感染病杂志》2013年第6期325-330,共6页Chinese Journal of Clinical Infectious Diseases

基　　金：国家自然科学基金，上海市松江区医学领先合作项目

摘　　要：目的 探讨发生与未发生抗结核药物的肝毒性(ATDH)患者血浆中差异性表达的微RNAs(miRNAs)分子。方法 采集3例ATDH和3例抗结核治疗无肝损伤(非ATDH)患者血浆样本,进行miRNAs芯片检测。对存在差异性表达的miRNAs分子,采用实时定量PCR进行验证。结果 与非ATDH患者相比,ATDH患者血浆中共筛选出12个差异性表达的miRNAs分子,其中表达上调的miRNAs有9个,表达下调的miRNAs有3个。实时定量PCR验证显示,ATDH患者血浆中显著上调的miRNAs有5个,包括miR-4655-3p、miR-1249、miR-1226-5p、miR-3620和miR-1224-5p,明显下调的miRNAs共3个,包括miR-7-5p、miR-424-5p和miR-584-5p。结论 ATDH与非ATDH患者之间在血浆内存在差异性表达的miRNAs分子。Objective To investigate the differential expression of miRNAs in plasma from patients with and without anti-tuberculosis drug-induced hepatotoxicity (ATDH).Methods The plasma samples were collected and subjected to miRNA microarray analysis from 3 ATDH patients and 3 non-ATDH patients.The differentially expressed miRNAs were verified using real-time quantitative PCR.Results Compared with non-ATDH patients,there were 12 miRNAs differentially expressed in ATDH patients,9 of which were up-regulated,and 3 down-regulated.By real-time quantitative PCR,5 up-regulated miRNAs,including miR-4655-3p,miR-1249,miR-1226-5p,miR-3620 and miR-1224-5p,3 down-regulated miRNAs,including miR-7-5p,miR-424-5p and miR-584-5p were verified in patients with ATDH.Conclusions There are miRNAs differentially expressed in plasma of patients with and without ATDH.

关 键 词：结核 肺 抗结核药 药物性肝损伤 微RNAS 

分 类 号：R575[医药卫生—消化系统]