液质联用法测定桃中三种杀菌剂的含量分布  

Distribution of three fungicides in peaches determined by liquid chromatography–tandem mass spectrometry

作　　者：徐小民[1] 何华丽[2] 吕美玲[3] 任一平[1] 韩见龙[1] 黄百芬[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051 [2]浙江工业大学化学工程与材料学院,杭州310014 [3]安捷伦科技(中国)有限公司,北京100102

出　　处：《食品安全质量检测学报》2013年第1期94-98,共5页Journal of Food Safety and Quality

基　　金：浙江省重点科技创新团队项目(2011R50021)~~

摘　　要：目的建立桃皮和肉中多菌灵、甲基硫菌灵和敌菌灵三种杀菌剂的液质联用(LC-MS/MS)测定方法,研究其在桃中的含量分布。方法桃皮和肉经分离后,分别用乙腈均质提取、PSA固相分散净化、C18高压液相色谱柱分离、MS/MS测定;分别计算皮和肉中三种杀菌剂的含量,研究三种杀菌剂在两者间的分布状况。结果方法检测限分别为0.1、0.2、10μg/kg;敌菌灵在样品中未检出,多菌灵和甲基硫菌灵在皮和肉中呈不同的含量分布特性。结论该方法灵敏度高、准确、操作简单,可用于桃中三种杀菌剂的含量分布研究。Objective To establish a method for the determination of three kinds of fungicides (carben-dazim, thiophanate-methyl and anilazine) in the peel and pulp of peaches by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and study their distributions in peaches. Methods The peel and pulp sepa-rated from peaches were extracted with acetonitrile by homogenization, purified by solid-phase dispersion ex-traction with PSA, separated by C18 HPLC column and measured by MS/MS, respectively. The concentrations of three kinds of fungicides in peel and pulp were calculated respectively. The results were used to study the distribution levels in peaches. Results The LODs were 0.1, 0.2 and 10 μg/kg for carbendazim, thiophan-ate-methyl and anilazine, respectively. Anilazine was not detected in samples. Different distribution results were found for carbendazim and thiophanate-methyl in peel and pulp. Conclusion The method is sensitive, accurate and easy to operate. It is suitable for the distribution study of these fungicides in peaches.

关 键 词：多菌灵 甲基硫菌灵 敌菌灵 液质联用 桃 含量分布 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]