高效液相色谱-四级杆/静电场轨道阱高分辨质谱研究人工“无抗奶”中青霉素类药物的降解产物  

Degradation products study of penicillins in artificial “non-anti-milk” by high performance liquid chromatography-linear trap quadrupole-orbitrap mass spectrometry

作　　者：赵凤娟[1] 岳振峰[1] 张毅[1] 秦晓红[1,2] 肖陈贵[1] 熊贝贝[1] 肖锋[1] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳市食品安全检测技术研发重点实验室,深圳518045 [2]深圳大学化学化工学院,深圳518045

出　　处：《食品安全质量检测学报》2014年第2期339-351,共13页Journal of Food Safety and Quality

基　　金：国家质检总局科技项目(2011IK236);深圳市基础研究项目(JC201105190972A)~~

摘　　要：目的研究人工"无抗奶"中青霉素类药物的降解产物。方法牛奶中的青霉素类药物经抗生素分解剂降解,采用乙腈-水(4:1,v:v)沉淀蛋白及提取后,采用高效液相色谱-四级杆/静电场轨道阱高分辨质谱对降解后的产物进行定性研究,确定残留标识物,并进行检测。结果几种青霉素类药物的残留标识物分别为相应的青霉噻唑酸及去羧青霉噻唑酸,对人工"无抗奶"的鉴别浓度低限为10μg/kg。结论此方法简单、快速、灵敏度高,可作为人工"无抗奶"的鉴别方法。Objective To study degradation products of penicillins in artificial“non-anti-milk”. Methods The penicillins in milk were borken down by β-Lactamases. After protein precipitation, the residues were ex-tracted with acetonitrile:water (4:1, v:v) solution, qualitatively detected by high performance liquid chromato-graphy-quadrupole/electrostatic field orbit trap high-resolution mass spectrometry. Results The residues of penicillins in artificial“non-anti-milk”were penicilloic acids and penilloic acids, and the limit of detection was 10μg/kg. Conclusion This method is simple, fast, and highly sensitive, and it is suitable for the identification of artificial“non-anti-milk”.

关 键 词：青霉素类药物 高效液相色谱-四级杆/静电场轨道阱高分辨质谱 降解产物 人工“无抗奶” 

分 类 号：O657.63[理学—分析化学] TS252.7[理学—化学]