光化学荧光法测定蔬菜中残留的氯菊酯  

Determination of permethrin in vegetable by photochemical-fluorimetry

作　　者：张媛[1] 丁茹[1] 孙笑笑[1] 李满秀[1] 

机构地区：[1]忻州师范学院化学系,忻州034000

出　　处：《食品安全质量检测学报》2014年第3期917-920,共4页Journal of Food Safety and Quality

基　　金：山西省高等学校大学生创新创业训练项目(2012334);忻州师范学院大学生科技创新项目(2013)~~

摘　　要：目的建立蔬菜中氯菊酯残留量的光化学-荧光分析方法。方法在pH 9.29的B-R缓冲溶液中,氯菊酯在紫外光照射下发生光化学反应,其荧光强度明显增强,激发波长为281 nm,发射波长为330 nm,用荧光分光光度计测定。结果氯菊酯在0.004～0.036μg/mL浓度范围内与荧光强度呈良好的线性关系,检出限为0.14ng/mL,相对标准偏差为1.0%。结论该方法灵敏度高,选择性好,适合蔬菜中氯菊酯残留量的测定。Objectives To establish a method for the determination of permethrin residues in vegetables by photochemical-fluorimetry. Methods Permethrin is a lower fluorescent substance, and its fluorescence in-tensity can be enhanced under the irradiation of ultraviolet light in B-R buffer medium (pH=9.29). The analyte had maximum excitation and emission wavelengths at 281 nm and 330 nm. Quantification was achieved by fluorimetric method. Results The linearity between fluorescence intensity and concentration of permethrin were in the range of 0.004～0.036μg/mL. The detection limit was 0.14 ng/mL. The relative standard deviation (RSD) was 1.0%. Conclusion The method has good sensitive and selectivity , it is suitable for the determina-tion of permethrin residues in vegetables.

关 键 词：氯菊酯 光化学 荧光分析法 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]