生菜中高效氯氰菊酯农残分析方法的优化  

Determination of cypermethrin pesticide in lettuce

作　　者：史少然 赵建庄[1] 范双喜[1] 吴昆明[1] 贾临芳[1] 梁丹[1] 

机构地区：[1]北京农学院农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206

出　　处：《食品安全质量检测学报》2014年第3期921-924,共4页Journal of Food Safety and Quality

基　　金：北京高校"青年英才计划"项目(FK2012-16);北京市教委科技面上项目"气质联机技术在农药残留降解菌筛选中的开发应用";人才强教深化计划--中青年骨干人才培养计划(PHR201108278);北京市教委面上科技项目-KM201210020014~~

摘　　要：目的建立生菜中高效氯氰菊酯农残分析方法。方法采用乙腈提取生菜中的高效氯氰菊酯,用Agilent弗罗里硅柱、NH2柱和C18柱固相萃取柱分别对样品进行净化,利用毛细管柱气相色谱法作为检测手段,GC-ECD进行定性分析。结果高效氯氰菊酯经过弗罗里硅柱的回收率在91.21%～102.78%,变异系数是0.63%～3.57%,最低检出限为0.001 mg·kg-1。结论高效氯氰菊酯顺式体氯氰菊酯和反式体氯氰菊酯2:3的双峰分离较好,操作简单,灵敏度高,添加回收率理想,可用于生菜中高效氯氰菊酯的残留检测分析。Objective To establish a method for determination of cypermethrin pesticide in lettuce. Methods Samples were extracted with acetonitrile, purified by Agilent SPE, Florisil column, NH2 column and C18 column, respectively, and detected by capillary column gas chromatography with ECD as detector. Results Recoveries of cypermethrin were 91.21%～102.78% with coefficient of variation of 0.63%～3.57%. The lowest detection limit was 0.001 mg·kg-1. Conclusion The method is simple, sensitive, accurate and re-producible, and the separation of cypermethrin cis-cypermethrin and trans-cypermethrin 2:3 bimodal peak is very satisfactory. This method can be used to detect residues of cypermethrin in lettuce.

关 键 词：高效氯氰菊酯 生菜 气相色谱法 优化 

分 类 号：O657.71[理学—分析化学] TS255.7[理学—化学]