瑞香狼毒中总酚酸含量测定  

Content Determination of Total Phenolic Acid in Stellera chamaejasme

作　　者：赵恒成[1] 穆红光 陈丽肖 甄攀[1] 

机构地区：[1]河北北方学院理学院,河北张家口075000 [2]河北宣化钢铁公司职工医院,河北宣化075100

出　　处：《河北北方学院学报（自然科学版）》2013年第4期12-14,20,共4页Journal of Hebei North University:Natural Science Edition

基　　金：河北省医学科学研究重点课题(20100480)

摘　　要：目的建立瑞香狼毒中总酚酸含量测定方法,并比较不同溶剂的提取效率。方法乙醚脱脂,70%乙醇提取;没食子酸为对照品;分光光度法测定总酚酸含量。结果标准曲线为A=1.8316C-0.0505,r=0.999 0,没食子酸含量在0.095~0.47μg·ml-1范围内呈良好线性关系,精密度RSD为1.6%,稳定性RSD为3.8%,回收率为99.47%。瑞香狼毒生药中总酚酸的含量为3.29 mg·g-1,提取物中总酚酸的含量为3.26mg·ml-1。结论 70%乙醇提取效率最高。测定方法简便、快速、灵敏、准确,可作为瑞香狼毒中总酚酸含量的测定方法。Objective To establish a method for content determination of total phenolic acid in Stellera chamaejasme,and to compare the extraction efficiencies of different solvents.Method Ether was used to degrease,70% ethanol was used to extract,spectrophotometry was to determine,and gallic acid was taken as the reference substance.Resutlt The standard curve was A=1.8316C-0.0505,r=0.9990,and the linear range was 0.095-0.47μg/ml.The RSD of the precision was 1.6%.The RSD of the stability was 3.8%.The mean recovery rate was 99.47%.The contents of total phenolic acid in Stellera chamaejasme and the extractive of 70%ethanol were 3.29mg/g and 3.26mg/ml,respectively.Conclusion The best solvent was 70% ethanol.This method is simple,accurate,sensitive and suitable for determining the total phenolic acid in Sterllera chamaejasme.

关 键 词：瑞香狼毒 总酚酸 没食子酸 分光光度法 

分 类 号：S[农业科学]