阿氏节杆菌菌株DA-1的厌氧反硝化研究  

Anaerobic Denitrification Characteristics of Arthrobacter arilaitensis DA-1

作　　者：张磊[1] 谈曙明[1] 缪鑫昕[1] 訾小利[1] 胡南[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211816

出　　处：《湖北农业科学》2013年第1期30-33,共4页Hubei Agricultural Sciences

基　　金：国家"973"计划项目(2009CB724700)

摘　　要：阿氏节杆菌(Arthrobacter arilaitensis)菌株DA-1是一株氨氮降解细菌,且在有氧及厌氧条件下均具有反硝化作用。通过对该菌的厌氧反硝化条件进行优化,考察了碳源、C/N、温度和初始pH对其反硝化作用的影响。结果表明,乙酸钠为最佳的反硝化碳源,当C/N为10∶1、温度为30℃、初始pH为7.0时,A.arilaitensis DA-1能获得最高的反硝化效率,在该条件下,向硝酸盐模拟污水(NO3--N浓度为25mg/L)中接入2%(V/V)的A.arilaitensis DA-1菌剂,经过48 h的静置培养,NO3--N的去除量达到了20.13 mg/L,而此时NO2--N积累浓度仅为0.56 mg/L。Arthrobacter arilaitensis DA-1 was an ammonia degradation strain,and showed denitrification ability under both aerobic and anaerobic conditions.The effects of carbon source,C/N,temperature and initial pH on anaerobic denitrification of A.arilaitensis DA-1 were investigated.The results showed that acetate was the best carbon source.When C/N,temperature and initial pH was 10∶1,30 ℃,and 7.0,respectively,the highest denitrification efficiency of A.arilaitensis DA-1 was obtained.Under the optimal conditions,inoculating A.arilaitensis DA-1 to simulated nitrate sewage containing 25 mg/L NO3-N with dose of 2%(V/V),after static culture of 48 h,the removal dose of NO3--N reached to 20.13 mg/L,while the accumulation of NO2--N was only 0.56 mg/L.

关 键 词：阿氏节杆菌(Arthrobacter arilaitensis) 反硝化作用 硝基氮 亚硝基氮 

分 类 号：S[农业科学]