亮菌产漆酶的液体和固体发酵条件优化  

Production of Laccase from Armillariella tabescens with Liquid and Solid Fermentation

作　　者：叶选怡[1] 杨丽红[2] 凌庆枝[3] 董丽辉[3] 何军邀[3] 张超[2] 

机构地区：[1]丽水学院生态学院,浙江丽水323000 [2]安徽理工大学医学院,安徽淮南232001 [3]浙江医药高等专科学校,浙江宁波315100

出　　处：《湖北农业科学》2013年第6期1410-1414,共5页Hubei Agricultural Sciences

基　　金：浙江省科技厅公益技术研究社会发展项目(2010C33072);浙江医药高等专科学校重点科研项目(ZPCSR2010001)

摘　　要：为了探讨液体和固体发酵条件对亮菌(Armillariella tabescens)产漆酶的影响,采用正交试验对亮菌液体和固体发酵产漆酶培养基和培养条件进行筛选。供试亮菌在液体培养时产漆酶甚微,在培养基和培养条件优化后最高酶活为7.92 U/mL;固体发酵时在以葡萄糖浓度0.6%、稻草麦麸质量比3∶7、温度27℃、含水量65%、接种量11 mL/100 g培养时漆酶酶活可达3 496.7 U/g。固体发酵的酶活显著高于液体发酵,固体发酵更适宜亮菌产漆酶。To study the effect of liquid and solid fermentation conditions on laccase production from Armillariella tabescens,the culture media and conditions for liquid and solid fermentation of A.tabescens was optimized through orthogonal test.Laccase activity of A.tabescens was very low by liquid fermentation;the highest enzyme activity was 7.92 U/mL.Under solid fermentation conditions,when mass ratio of straw and bran was 3∶7,temperature was 27 ℃,water content was 65%,inoculum size was 11%,the enzyme activity reached up to 3 496 U/g,which was significantly higher than that of liquid fermentation.The solid fermentation was more suitable for laccase production by A.tabescens fermentation.

关 键 词：亮菌(Armillariella tabescens) 漆酶 液体发酵 固体发酵 

分 类 号：S[农业科学]