不同地理群体的鲇线粒体DNA 12S rRNA基因序列与遗传结构分析  

Analysis on Sequence and Genetic Structure of Mitochondrial DNA 12S rRNA of Silurus asotus from Different Geographical Populations

作　　者：王庆容[1] 曾燕玲[1] 杨秀荣[1] 

机构地区：[1]遵义师范学院生物系,贵州遵义563002

出　　处：《湖北农业科学》2013年第11期2603-2606,共4页Hubei Agricultural Sciences

基　　金：贵州省科技厅项目(黔科合J字2011-2189)

摘　　要：对长江中上游主要支流5个野生群体的鲇(Silurus asotus)——乌江群体(WJ)、雅砻江群体(YLJ)、岷江群体(MJ)、金沙江群体(CS)、舞阳河群体(WYH)线粒体DNA 12S rRNA基因序列与遗传结构进行分析,27个样品的线粒体DNA 12S rRNA基因进行PCR扩增及测序,并进行序列比对。结果表明,共检测出36个变异位点,8种单倍型。乌江、雅砻江、岷江、金沙江、舞阳河5个野生群体的单倍型多样性(H)分别为0.857、0.600、0.600、0.667、0.400;5个野生群体的核苷酸多样性(π)分别为0.014 20、0.000 63、0.000 63、0.014 02、0.000 42。舞阳河群体存在负向选择或种群扩张,其余群体符合中性进化模型,只有金沙江群体的遗传差异显著。对5个群体进行分子变异等级分析,群体间分子变异不显著。乌江、雅砻江、岷江、金沙江群体之间的亲缘关系较近,舞阳河群体与其他群体间的亲缘关系较远。The aim of the present study was to survey mtDNA 12S rRNA sequence variation to assess genetic diversity and genetic structure of the wild Silurus asotus in the upstream and midstream of Yangtze River that included the Wujiang River(WJ),Yalong Jiang(YLJ),Min River(MJ),Chin-sha River(CS),and Wuyanghe(WYH).The PCR technique was used to amplify the mtDNA 12S rRNA among 27 samples.The PCR products were sequenced and blasted.The results showed that 8 haplotypes and 36 variable sites were detected among all mitochondrial 12S rRNA sequences of 27 samples.Haplotype diversities(H) in the Wujiang River(WJ),Yalong Jiang(YLJ),Min River(MJ),Chin-sha River(CS) and Wuyanghe(WYH) wild populations were 0.857,0.600,0.600,0.667 and 0.400 respectively.Nucleotide diversities(π) of the five wild population were 0.014 20,0.000 63,0.000 63,0.014 02 and 0.000 42 respectively.Wuyanghe(WYH) populations existed negative selecting or population expanding,the other groups fited to neutral evolution model,and all the other populations showed no strong genetic differences except Chin-sha River(CS) population.There were no significant genetic differentiation among all five populations.Wujiang River(WJ),Yalong Jiang(YLJ),Min River(MJ),and Chin-sha River(CS) putative populations had the closest relation among the five populations,the Wuyanghe(WYH) population had the greatest genetic distance among the five populations.

关 键 词：鲇(Silurus asotus) 线粒体DNA 12S RRNA 遗传多样性 

分 类 号：S[农业科学]