桂林市公园苏云金芽孢杆菌菌株的分离  

Isolation of Bacillus thuringiensis from parks in Guilin

作　　者：朱桂梅[1] 刘建国[1] 龙施华[1] 郝再彬[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541006

出　　处：《湖北农业科学》2013年第17期4107-4109,共3页Hubei Agricultural Sciences

基　　金：国家高技术研究发展计划项目(2008ZX08001-001);国家重点基础研究发展计划项目(2009CB118902);国家自然科学基金项目(31060193)

摘　　要：从桂林市部分公园采集了80份土样,分离得到11株苏云金芽孢杆菌,并对其杀虫晶体蛋白的形态及表达情况进行了研究。通过光学显微镜观察,发现这些菌株产生的伴孢晶体有菱形、球形、不规则形等。利用SDS-PAGE对蛋白进行分析,结果表明9株苏云金芽孢杆菌表达的蛋白大小约为135 ku,5株表达蛋白大小约65 ku,1株表达蛋白大小约90 ku,1株表达蛋白大小约45 ku,5株同时表达约135 ku和65 ku两种蛋白。产小菱形晶体的菌株7-4可能对美国白蛾有高毒力,值得进一步深入研究。11 Bacillus thuringiensis(Bt) isolates were obtained from 80 soil samples collected from some parks in Guilin,and the morphology and expression of insecticidal crystal proteins from these strains were futher studied.The optical microscopic observation showed that there were bipyramidal,spherical,and irregular parasporal crystals in the 11 Bt isolates.Analysis of proteins by SDS-PAGE indicated that 9 Bt isolates could express proteins with 135 ku molecular mass,5 strains with 65 ku,1 strain with 90 ku,1 strain with 45 ku,and 2 isolates expressed both 130 ku and 60 ku proteins.Strain 7-4 producing small bipyramidal crystal protein may have high toxcity to Hyphantria cunea(Drury),which deserved to be further studied.

关 键 词：苏云金芽孢杆菌 伴孢晶体 光学显微观察 SDS-PAGE 

分 类 号：S[农业科学]