小鼠卵巢玻璃化冻存过程中FSH干预对VEGF及VEGFR-2表达的影响  

The Influence of FSH Interpose on the VEGF and VEGFR-2 Expression in the Different Stage of Mouse Ovary Vitrification

作　　者：陈杰[1,2] 张宁[1] 王燕蓉[1,2] 裴秀英[1] 黑常春[1,2] 马会明[1,2] 常青[1,2] 马文智[1,2] 蔡玉芳[1,2] 孙苗[1] 

机构地区：[1]宁夏医科大学生育力保持教育部重点实验室,宁夏生殖与遗传重点实验室,银川750004 [2]宁夏医科大学人体解剖与组织胚胎学系,银川750004

出　　处：《宁夏医科大学学报》2013年第3期236-239,243+228,共6页Journal of Ningxia Medical University

基　　金：国家自然科学基金资助项目(81160085);宁夏回族自治区自然科学基金重点项目(NZ1084);宁夏教育厅重点项目(宁教高[2011]263)

摘　　要：目的通过在玻璃化冻存不同时段给予小鼠卵巢组织重组人促卵泡刺激素(rFSH)干预,观察rFSH对VEGF以及VEGFR-2表达的影响,探索卵巢玻璃化冻存过程中最佳的FSH保护途径。方法将4周龄C57BL/6J小鼠卵巢分为新鲜对照组(FCG)、玻璃化冻存对照组(VCG)、0.3IU.mL-1FSH干预玻璃化冻存组,包括全程添加FSH组(OG-FSH)、冻存前添加FSH组(BG-FSH)以及冻存后添加FSH组(AG-FSH)。通过形态学技术观察正常卵泡百分比;采用免疫组织化学技术观察卵巢不同细胞VEGF以及VEGFR-2的表达;通过Western-blot方法观察并分析不同玻璃化冻存时段的FSH干预对卵巢VEGF以及VEGFR-2蛋白表达量的影响。结果正常卵泡百分比、VEGF及VEGFR免疫组织化学和Western-blot检测结果组间比较差异均有统计学意义。其中,OG-FSH组各项检测指标均高于其他各组(P<0.05),且BG-FSH、AG-FSH组与FCG相似,高于VCG组(P<0.05)。结论玻璃化冻存全程添加FSH干预有利于提高正常卵泡数,以及VEGF、VEGFR-2蛋白的表达。Objective To investigate the best protection way of FSH,which interposed in different stage of vitrification process via observing the expression of VEGF and VEGFR-2.Methods:4-week-old C57BL/6J mice were divided into fresh control group(FCG),vitrification control group(VCG) and the vitrification intervention group by 0.3IU·mL-1 FSH(VIG).VIG included the group of FSH interposed in the overall process of vitrification group(OG-FSH),FSH interposed in the before process of vitrification group(BG-FSH) and FSH interposed in the after process of vitrification group(AG-FSH).The percentage of normal follicles were observed through morphology and,the expression of VEGF,VEGFR-2 in different cells of ovary were observed by immunohistochemical technique.The effect on the expression of VEGF,VEGFR-2 protein about FSH intervention in different stage of vitrification was observed and analyzed by western-blot.Results Normal follicles,VEGF and VEGFR-2.in OG-FSH were significantly higher than those in other groups(P&lt;0.05).Conclusion OG-FSH is in favour of improving the percentage of normal follicles and the expression of VEGF,VEGFR-2 protein.

关 键 词：小鼠 卵巢 FSH 玻璃化冻存 VEGF VEGFR-2 

分 类 号：R737.3[医药卫生—肿瘤]