大鼠胶质瘤细胞中生物钟基因Period2的生物节律性研究  

The Biological Rhythms of CircadianClock Gene Period2 in Glioma Cells

作　　者：李张珂[1,2] 李延辉[1] 王凡[3] 尹磊[1] 牛占峰[4] 夏鹤春[4,2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏颅脑疾病重点实验室,银川750004 [3]荆门市第一人民医院,荆门448000 [4]宁夏医科大学总医院,银川750004

出　　处：《宁夏医科大学学报》2013年第5期485-488,496,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(30860289)

摘　　要：目的揭示生物钟基因Period2(Per2)在鼠胶质瘤C6细胞内周期表达规律。方法采用12-十四酸佛波酯-13-乙酸盐(PMA)分别刺激体外培养的大鼠胶质瘤C6及NIH3T3细胞,在ZT0-ZT48之间设14个时间点,最初PMA的刺激时间记为ZT0,均在PMA刺激后每隔4h分别提取C6及NIH3T3细胞总RNA。利用标准曲线法Real-time PCR检测相应时间点Per2的mRNA的表达水平。结果 C6细胞中生物钟rPer2基因表达最高点在ZT28,最低点在ZT16;NIH3T3细胞中生物钟mPer2基因表达最高点在ZT24,最低点在ZT12,两者均呈现明显的节律性;C6细胞与NIH3T3细胞同时间点Per2基因表达差异均有统计学意义(均P<0.001)。结论经PMA诱导后,Per2在C6及NIH3T3细胞均呈节律性表达,但二者表达节律存在明显差异,可作为研究生物种基因与胶质瘤的时间生物治疗的理论基础。Objective To investigate the rhythm expression of circadian gene Period2(Per2) in glioma cells in vitro.Methods The cultured C6 glioma cells and NIH3T3 cells were stimulated by PMA.14 time points were set between ZT0-ZT48 and initial PMA stimulation time was recorded as ZT0.Total RNA of C6 and NIH3T3 cells were extracted after PMA was stimulated every 4h.mRNA expression levels of the corresponding time points Per2 were detected with the Real-time PCR.Results The highest point of the biological clock rPer2 gene expression in C6 cells was ZT28 while the lowest point of the biological clock was ZT16;The highest point of the biological clock mPer2 gene expression in NIH3T3 cells was ZT24 while the lowest point of the biological clock was ZT12.Per2 gene expression showed significant difference in C6 cells and NIH3T3 cells at the same time point(all P&lt;0.001).Conclusion PMA can induce the circadian oscillation of Per2 in both of C6 and NIH3T3 cells,but there are significant differences in the expression of rhythm.This can be used as theoretical basis of chronotherapy of gliomas.

关 键 词：PER2 胶质瘤 生物节律性 

分 类 号：R739.4[医药卫生—肿瘤]