不同病程糖尿病大鼠视网膜病变的研究  

The Pathological Change of Retina of Diabetic Rats with Different Courses

作　　者：李小璐 马雅玲[2] 

机构地区：[1]宁夏人民医院宁夏眼科医院,银川750011 [2]宁夏医科大学总医院眼科,银川750004

出　　处：《宁夏医科大学学报》2013年第8期890-893,954,共5页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ09138)

摘　　要：目的观察不同时间点糖尿病大鼠视网膜病理结构及功能的改变,探讨糖尿病大鼠视网膜病理变化与病程的关系。方法取健康雄性SD大鼠64只,随机分为正常对照组(CON组),糖尿病1个月组(DM1组)、3个月组(DM2组)、5个月组(DM3组),糖尿病组实验前用链脲佐菌素(STZ)制造糖尿病大鼠模型。随机抽取相应DM1、DM2、DM3组及正常对照组大鼠,用透射电镜观察不同时间点的糖尿病大鼠和正常大鼠视网膜的超微结构,视网膜HE染色切片、伊文思蓝(EB)视网膜铺片及视网膜EB含量测定观察视网膜结构及功能的变化。结果病程初期糖尿病大鼠视网膜色素上皮细胞、膜盘、外核层、内核层及神经节细胞等超微结构已出现改变,且病变随病程进展而加重。视网膜HE染色示CON组视网膜未见明显异常,DM1、DM2、DM3组视网膜各层组织病理改变随病程延长而加重。EB染色视网膜铺片显示于糖尿病3个月时已出现血管荧光渗漏,5个月时视网膜血管出现结构明显不规则和大量荧光渗漏。视网膜EB含量随糖尿病病变加重而不断增多,CON组、DM1组、DM2组、DM3组视网膜EB含量分别为(0.227±0.051)、(0.325±0.054)、(0.510±0.027)、(0.721±0.035)ng·mg-1,差异有统计学意义(P<0.05)。结论糖尿病大鼠视网膜结构在1个月已出现病变,且随病程延长而加重。Objective To investigate the pathological change of retina of diabetic rats with different courses and to explore the relation between pathological change of retina in diabetic rats and disease course. Methods 64 male SD rats were divided randomly into the normal control group( CON group),the diabetic group at 1month( DM1 group),the diabetic group at 3 months( DM2 group),the diabetic group at 5 months( DM3group). The diabetic models were induced by intraperitoneally injection of streptozotocin( STZ). Rats in DM1group,DM2 group,DM3 group and control group were selected respectively and the ultrastructure was bserved by transmission electron microscope. Pathological change of retinal vessels was investigated through HE stain and retinal stretched preparation with Evans blue( EB),calculating retinal EB content. Results The pigment epithelial cell,membranous disc,out nuclear layer,inner nuclear layer and gangliocyte had changed at 1 month after modeling,and the pathology aggravated as the disease developed. The HE staining showed a similar trend of retinal pathology. The retinal stretched preparation with EB showed that fluorescence leakage had appeared in retina of DM3 group. The retinal vascular became highly irregular and had a lot of fluorescence leakage in DM3 group. The EB content in retinal increased with disease aggravated. Retinal EB content in CON group,DM1 group,DM2 group,DM3group was( 0. 227 ± 0. 051),( 0. 325 ± 0. 054),( 0. 510 ±0. 027),( 0. 721 ± 0. 035) ng·mg-1and difference among groups showed statistical significant( P &lt; 0. 05).Conclusion The retinal pathology had appeared in early of diabetes mellitus,and aggravated with the development of diabetic disease.

关 键 词：糖尿病性视网膜病变 伊文思蓝 视网膜铺片 血-视网膜屏障 

分 类 号：R774.1[医药卫生—眼科]