MMP-1/TIMP-2在子宫内膜异位症大鼠子宫内膜上的表达  

Expression of MMP-1/TIMP-2 in endometrium of endometriosis in rats

作　　者：张登霞[1] 杨雪萍[2] 

机构地区：[1]兰州大学第一医院妇产科,甘肃兰州730000 [2]甘肃省人民医院妇产科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2013年第3期1-3,共3页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的检测基质金属蛋白酶-1(MMP-1)/组织金属蛋白酶抑制物-2(TIMP-2)在子宫内膜异位症(EMS)大鼠在位、异位病灶中的表达,探讨其在EMS子宫内膜中表达的特点、相关关系及影响。方法选取健康未孕雌性大鼠30只,从中随机抽取10只作为空白组,不做任何处理;其余30只采用移植法建EMS模型,建模后4周2次手术开腹,大体观察并组织病理学检查确定建模成功后,取在位内膜组织大鼠10只(在位组)、异位内膜组织大鼠10只(异位组),通过免疫组化法测定子宫内膜中MMP-1/TIMP-2的表达。结果异位组与空白组、在位组比较,MMP-1表达高,TIMP-2表达低,差异具有统计学意义(P<0.05);空白组、在位组MMP-1低表达,TIMP-2高表达,差异无统计学意义。结论 MMP-1/TIMP-2平衡的破坏及在EMS大鼠异位内膜组织的异常表达,可能参与了EMS的病理发生与发展。Objective To study the expression of matrix metalloproteinases-1(MMP-1)/tissue inhibitor of metalloproteinase-2(TIMP-2) in endometriosis in orde to investigate the characteristics,the relationship and influence expression of MMP-1/TIMP-2.Methods 10 healthy non-pregnant female rats were selected randomly as the blank group,without any treatment.The remaining30 were treated by transplant method to build model of endometriosis.The second laparotomy operation modeling was made after 4 weeks.Gross observation and histological examination were confirmed after the success of modeling for the eutopic endometrial tissues of 10 rats(in-group)and ectopic endometrial tissue of 10 rats(ectopic group).Results MMP-1 expression of ectopic group was higher than that of the blank group,and in-group,while their expression of TIMP-2was lower.It had statistical significance,P&lt;0.05.There was no significant difference for the low expression of MMP-1 and high expression of TIMP-2 in the blank group and in-group.Conclusion The damages of the balance of MMP-1/TIMP-2 and the abnormal expression of eutopic and ectopic endometrium in endometriosis rat models may involve in the occurrence and developmentof tissue pathology for endometriosis.

关 键 词：子宫内膜异位症 大鼠 基质金属蛋白酶-1 组织金属蛋白酶抑制物-2 

分 类 号：R[医药卫生]