鸡肉中弯曲菌定量检测方法的建立与初步应用  

Development and application of a quantitative detection of Campylobacter spp.in chicken meat

作　　者：张小燕 翟伟华 朱佳琪 黄金林 焦新安 

机构地区：[1]扬州大学江苏省人兽共患病学重点实验室,江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《食品安全质量检测学报》2014年第6期1673-1678,共6页Journal of Food Safety and Quality

基　　金：国家自然科学基金(31372449);国家科技支撑计划(2014BAD13B02);江苏高校优势学校建设工程项目;江苏省普通高校研究生科研创新计划项目(CXLX13_922)~~

摘　　要：目的参照国标方法,建立鸡肉中弯曲菌定量检测的CCDA平板计数法。方法通过对选择性CCDA培养基中添加不同浓度组合的抗生素和生长促进剂,确定最佳添加浓度组合,并与普通CCDA平板计数法进行比较,建立鸡肉中弯曲菌定量检测方法,同时评价该方法的特异性和检测限。结果添加一定浓度抗生素和生长促进剂的选择性CCDA培养基和普通CCDA培养基对弯曲菌标准菌株定量计数的吻合度为(100±5)%;除弯曲菌外,其他5种肠道细菌在该CCDA培养基中均不能生长;该方法对弯曲菌菌悬液可检测至10 CFU/mL。运用该方法对50份鸡肉样品进行调查研究,共检测出22份阳性样品,弯曲菌检出率为44%,平均检测值为20CFU/100 cm^2,检测结果与国标方法阳性符合率为100%。结论该方法简便、特异性好、准确度高,为定量检测鸡肉中弯曲菌提供了新技术。ABSTRACT:Objective A quantitative detection method was developed to detect Campylobacter spp. con-tamination in chicken meat. Methods According to the methods of national standards, a kind of selective CCDA medium which contains antibiotics and growth promoter was selected and modified to establish a quan-titative method for the detection of Campylobacter spp.. Results The conformity of the numbers of Campy-lobacter in selective CCDA medium and conventional CCDA medium was (100±5)%. The specific results showed that no other bacteria strains could be cultured in this selective CCDA medium except Campylobacter;the limit of detection was as few as 10 CFU/mL sample. In this way, 50 chicken meat samples were investi-gated for Campylobacter. The accuracy of this method was shown to be 100%when compared to GB/T 4789. 9-2008. Conclusion The method was simply operated with high accuracy which was proved to be practical for detection of Campylobacter spp..

关 键 词：弯曲菌 定量检测 生长促进剂 抗生素 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]