人肝癌细胞培养中CCK-8法与BrdU-ELISA法检测细胞增殖的比较  

A comparison of CCK-8 and BrdU-ELISA for the proliferation of human hepatic cancer cell line HepG2

作　　者：张大伟[1] 邢雪[2] 邓乃梅[2] 谭雪莹[2] 汤海涛[2] 

机构地区：[1]青岛大学医学院,山东青岛266000 [2]青岛市市立医院肝胆外科,山东青岛266000

出　　处：《临床普外科电子杂志》2013年第4期4-7,共4页Journal of General Surgery for Clinicians(Electronic Version)

摘　　要：目的比较CCK-8法与BrdU-ELISA法检测人肝癌HepG2细胞增殖的可靠性。方法待细胞贴壁生长融合度分别达60%及80%时,以10-7mol/L浓度AngⅡ刺激细胞增殖,24小时后分别用CCK-8法和BrdU-ELISA法,以及免疫荧光方法检测细胞增殖情况。结果细胞60%汇合时CCK-8检测组和BrdU-ELISA检测组与各自的对照组比较,比值分别为1.30和1.59,检测值近似;细胞80%汇合时二者分别为1.02和1.40,免疫荧光结果显示AngⅡ刺激组的增殖细胞明显高于对照组。结论与CCK-8法相比,BrdU-ELISA更能客观地反映细胞增殖情况。Objective To compare the reliability between CCK-8 method and BrdU ELISA method in measure the proliferation of human hepatic cancer cell lines HepG2. Methods Perform CCK-8 and BrdU-ELISA method, as well as immunofluorescence method to examine the the proliferation of cells stimulated by using Ang II with concentration of 10-7mol/L 24 hours when the degree of the cell confluence reaches 60%and 80%. Results The ratio are respectively 1.30 and 1.59 comparing with their own control group respectively by CCK-8 and BrdU-ELISA method under the circumstance that the cell confluence reaches 60%which have an approximate estimated value;and the ratio are 1.02 and 1.40 when the cell confluence reaches 80%which the immunofluorescence method reveals that those of the group with stimulation of Ang II have a obviously higher cell proliferation than control group. Conclusion BrdU-ELISA method could present a more objective situation of the cell proliferation compared with CCK-8 method.

关 键 词：CCK-8检测 5-溴脱氧尿嘧啶核苷-ELISA检测 血管紧张素Ⅱ 细胞增殖 

分 类 号：R73[医药卫生—肿瘤] R28[医药卫生—临床医学]