一种从培养上清获得高滴度腺病毒的方法  

A NEW WAY TO INCREASE THE TITE OF ADENOVIRUSES FROM CUITURE

作　　者：杨世昕[1,2] 吴军[1,2] 罗高兴[1,2] 易绍萱 贺伟峰[1,2] 黄赤兵 周立新[1,2] 李彦 张宁[1,2] 陈希炜 张小蓉[1,2] 

机构地区：[1]第三军医大学西南医院烧伤研究所 [2]重庆市器官移植基础研究所,重庆400038

出　　处：《中国医学生物技术应用》2004年第1期39-41,共3页The Chinese Academic Medical Magazine of Organisms

摘　　要：目的:寻找一种有效的提高腺病毒的滴度而又不损害其活性的方法。方法:腺病毒原夜升化冻干(sublimation drying)后溶解稀释,通过斑点形成实验、免疫组化检验其感染活性。结果:升化冻干后稀释的腺病毒液感染滴度达到4.4××1012pfu。结论:冻干、稀释是一种提高腺病毒滴度的有效方法。OBJCTIVE: To serch a new way to improve the tite of adenoviruses without getting down itsaffection capacity, METHODS:The primary succus of adenoviruses was condensed by sublimation drying anddilution. Its affection was determined by examination of plaque-forming units and imunohistochemical assay.RESULTS:The tite of adenoviruses can reach as high as 4.4×1012pfu. CONCLUSION:Sublimation dryingand dilution is a new and efficient way to improve the tite of adenoviruses.[

关 键 词：冻干 滴度 腺病毒 

分 类 号：R373[医药卫生—病原生物学]