Polymorphisms in programmed death-1 gene are not associated with chronic HBV infection in Chinese patients  

作　　者：Feng Lv,Yu-Feng Gao,Zhen-Huan Zhang,Ming-Fang Cui,Shu-Ling Xia,Xu Li,Hua-Fa Yin,Department of Infectious Diseases,the First Affiliated Hospital,Anhui Medical University,Hefei 230022,Anhui Province,China Tian-Chen Zhang,Fa-Ming Pan,Department of Epidemiology and Biostatistics,School of Public Health,Anhui Medical University,Hefei 230032,Anhui Province,China Author 

出　　处：《World Journal of Hepatology》2011年第3期72-78,共7页世界肝病学杂志（英文版）（电子版）

基　　金：Supported by the Grants from the National Natural Science Foundation of China, No.30700698 and 30771907;the Foundation of Pre-973 Program Projects, No. 2009CB526411

摘　　要：AIM:To investigate the association between the programmed death-1(PD-1) polymorphisms and genetic susceptibility of chronic hepatitis B virus(HBV) infection in Chinese patients.METHODS:Two single nucleotide polymorphisms(SNPs),PD-1.1 G > A and PD-1.2 G > A,were genotyped in 539 patients with chronic HBV infection and 353 other family members(HbsAg-) from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay.The associations between PD-1 polymorphisms and genetic susceptibilityof chronic HBV infection were analyzed usng the familybased association analysis method.RESULTS:No association or linkage was detected among 539 patients.Univariate(single-marker) familybased association tests demonstrated that PD-1 genotypes,alleles and transmitted haplotypes are not associated with chronic HBV infection(all with P value more than 0.05).Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring(P = 0.688880,P = 1.000000 respectively).CONCLUSION:The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.AIM: To investigate the association between the programmed death-1(PD-1) polymorphisms and genetic susceptibility of chronic hepatitis B virus (HBV) infection in Chinese patients.METHODS: Two single nucleotide polymorphisms (SNPs), PD-1.1 G > A and PD-1.2 G > A, were genotyped in 539 patients with chronic HBV infection and 353 other family members (HbsAg-) from 256 nuclear families using polymerase chain reactiorestriction fragment length polymorphisms assay. The associations between PD-1 polymorphisms and genetic susceptibility of chronic HBV infection were analyzed usng the family-based association analysis method.RESULTS: No association or linkage was detected among 539 patients. Univariate (single-marker) family-based association tests demonstrated that PD-1 genotypes, alleles and transmitted haplotypes are not associated with chronic HBV infection (all with P value more than 0.05). Transmission/disequilibrium test and sibship disequilibrium test analysis showed no excess of the alleles from heterozygous parents to affected offspring (P = 0.688880, P = 1.000000 respectively).CONCLUSION: The data demonstrated that PD-1.1 and PD-1.2 polymorphisms are not associated with chronic HBV infection in Chinese patients.

关 键 词：Programmed death-1 HEPATITIS B virus Single NUCLEOTIDE POLYMORPHISM Genetic ASSOCIATION study FAMILY-BASED ASSOCIATION test 

分 类 号：R512.62[医药卫生—内科学]