CYP2E1 immunoglobulin G4 subclass antibodies after desflurane anesthesia  

作　　者：Chrysanthi Batistaki George Michalopoulos Paraskevi Matsota Tzortzis Nomikos Konstantinos Kalimeris Maria Riga Maria Nakou Georgia Kostopanagiotou 

机构地区：[1]2~(nd) Department of Anesthesiology, School of Medicine, University of Athens, "Attikon" University Hospital, 12462 Athens, Greece [2]Department of Gastroenterology, "Tzaneion" Peripheral General Hospital, 18536 Piraeus, Greece [3]Department of Nutrition and Dietetics, Harokopio University, 17671 Athens, Greece

出　　处：《World Journal of Hepatology》2014年第5期340-346,共7页世界肝病学杂志（英文版）（电子版）

摘　　要：AIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were > 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student's t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher's exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P < 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P > 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and length of hospiAIM: To investigate CYP2E1 IgG4 autoantibody levels and liver biochemical markers in adult patients after anesthesia with desflurane. METHODS: Forty patients who were &gt; 18 years old and undergoing elective surgery under general anes-thesia with desflurane were studied. Alpha-glutathione-S-transferase(aGST) and IgG4 antibodies againstCYP2E1 were measured preoperatively and 96 h post-operatively, as well as complete blood count, prothrom-bin time(PT), activated partial thromboplastin time(aPTT), international normalized ratio(INR), aspartate aminotransferase(SGOT), alanine aminotransferase(SGPT), g-glutamyl-transpeptidase(gGT), alkaline phosphatase, total serum proteins, albumin and bili-rubin. A separate group of 8 patients who received re-gional anesthesia was also studied for calibration of the methodology used for CYP2E1 IgG4 and aGST measure-ments. Student’s t-test and the Mann-Whitney U test were used for comparison of the continuous variables, and Fisher’s exact test was used for the categorical variables. All tests were two-tailed, with statistical sig-nificance set as P &lt; 0.05.RESULTS: None of the patients developed postopera-tive liver dysfunction, and all patients were successfully discharged from the hospital. No statistically significant difference was observed regarding liver function tests(SGOT, SGPT, γGT, bilirubin, INR), aGST and CYP2E1 IgG4, before and after exposure to desflurane. After dividing patients into two subgroups based on whether or not they had received general anesthesia in the past, no significant difference in the levels of CYP2E1 IgG4 was observed at baseline or 96 h after desflurane administration(P = 0.099 and P = 0.051, respectively). Alpha-GST baseline levels and levels after the interven-tion also did not differ significantly between these two subgroups(P &gt; 0.1). The mean aGST differences were statistically elevated in men by 2.15 ng/mL compared to women when adjusted for BMI, duration of anesthe-sia, number of times anesthesia was administered pre-viously and le

关 键 词：Drug-induced-liver injury DESFLURANE ANESTHESIA HEPATOTOXICITY CYP2E1 IGG4 

分 类 号：R614[医药卫生—麻醉学]