Fourier transform infrared spectroscopy of gallbladder carcinoma cell line  

作　　者：Du, Jun-Kai Shi, Jing-Sen Sun, Xue-Jun Wang, Jian-Sheng Xu, Yi-Zhuang Wu, Jin-Guang Zhang, Yuan-Fu Weng, Shi-Fu 

机构地区：[1]Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Gen Surg, Xian 710061, Peoples R China [2]Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

出　　处：《Hepatobiliary & Pancreatic Diseases International》2009年第1期75-78,共4页国际肝胆胰疾病杂志（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China(No.30500119)

摘　　要：BACKGROUND: Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line. METHODS: The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF(2) window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue. RESULTS: Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue. CONCLUSION: The diagnosis of carcinoma tissue by FTIR spectroscopy has a sufficient cellular basis.BACKGROUND: Fourier transform infrared (FT-IR) spectroscopy is a physical method applied to the study of cellular changes at the molecular level in various normal and diseased human tissues, including cancer. This study was undertaken to establish a cellular basis for the diagnosis of carcinoma tissue, using FT-IR spectroscopy to study a carcinoma cell line and investigating the specific spectral features of the cell line. METHODS: The FT-IR spectra of cultured gallbladder carcinoma cells (GBC-SD) smeared on a BaF(2) window were measured with a Nicolet Magna750-II FT-IR spectrometer. A comparative study was subsequently carried out between the spectra of cultured gallbladder carcinoma cells and those of corresponding carcinoma tissue. RESULTS: Several infrared spectral features were obtained, and the results suggest that the spectral features of the carcinoma cell line reflect those of carcinoma tissue, though the latter are more complex, probably due to the intrinsic complexity of the tissue. CONCLUSION: The diagnosis of carcinoma tissue by FTIR spectroscopy has a sufficient cellular basis.

关 键 词：spectroscopy  Fourier transform infrared gallbladder neoplasm cell line 

分 类 号：R735.8[医药卫生—肿瘤]