Enzymes activity of intestinal grafts after liver small bowel transplantation in rats  

作　　者：Xiao-Yi Sun Guo Wang Hui-Fen Shi Ming-Fa Wei Guang-Fu Yin Li-Qiang Ru the Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College Department of Neurobiology, School of Basic Medical Sciences Huazhong University of Science and Technology, Wuhan 430030, China 

出　　处：《Hepatobiliary & Pancreatic Diseases International》2003年第3期338-343,共6页国际肝胆胰疾病杂志（英文版）

基　　金：This study was supported by the National Nature Science Foundation of China (No. 393700597) and Science Foundation of Ministry of Health, China (No. 0733-501).

摘　　要：OBJECTIVES: To investigate the activity alterations of enzymes in intestine grafts after liver/small bowel transplantation in rats and the relations of these changes to immune rejection of grafts. METHODS: A model of liver/small bowel transplantation (LSBT) was established in closed colony SD and Wistar rats. The activity of enzymes including triphosphatase (ATPase), alkalinophosphatase (AKP), acytelcholinesterase (AchE), oxidesynthase (NOS) and monoamine oxidase (MAO) in bowel grafts was studied histochemically at regular postoperative intervals. RESULTS: The activity of enzymes in the wall of the grafts disappeared eventually in isolated small bowel transplantation (SBT) rats. In contrast, the activity in LSBT rats remained and recovered postoperatively. CONCLUSIONS: The rejection in grafted intestine could be prevented or delayed in LSBT rats. The changes in the activity of enzymes and neurons might be used to detect the rejection and function of the graft.OBJECTIVES: To investigate the activity alterations of enzymes in intestine grafts after liver/small bowel transplantation in rats and the relations of these changes to immune rejection of grafts. METHODS: A model of liver/small bowel transplantation (LSBT) was established in closed colony SD and Wistar rats. The activity of enzymes including triphosphatase (ATPase), alkalinophosphatase (AKP), acytelcholinesterase (AchE), oxidesynthase (NOS) and monoamine oxidase (MAO) in bowel grafts was studied histochemically at regular postoperative intervals. RESULTS: The activity of enzymes in the wall of the grafts disappeared eventually in isolated small bowel transplantation (SBT) rats. In contrast, the activity in LSBT rats remained and recovered postoperatively. CONCLUSIONS: The rejection in grafted intestine could be prevented or delayed in LSBT rats. The changes in the activity of enzymes and neurons might be used to detect the rejection and function of the graft.

关 键 词：small bowel transplantation combined liver/small bowel transplantation ENZYME 

分 类 号：R656[医药卫生—外科学]