Effects of xenogeneic adipose-derived stem cell transplantation on acute-on-chronic liver failure  

作　　者：Wei Zhu Xiao-Lei Shi Jiang-Qiang Xiao Guang-Xiang Gu Yi-Tao Ding Zheng-Liang Ma 

机构地区：[1]The Combination of Chinese Medicine with Western Medicine Gulou Clinical Medical College,Nanjing University of Chinese Medicine [2]Department of Anesthesiology,Affiliated Drum-Tower Hospital,Nanjing University Medical School [3]Department of Hepatobiliary Surgery,Affiliated Drum-Tower Hospital,Nanjing University Medical School [4]Jiangsu Province's Key Medical Center for Hepatobiliary Disease

出　　处：《Hepatobiliary & Pancreatic Diseases International》2013年第1期60-67,共8页国际肝胆胰疾病杂志（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China (30901431)

摘　　要：BACKGROUND:Adipose-derived stem cells(ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure(ACLF) This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.METHODS:ADSCs isolated from porcine fat tissue were expanded and labeled with BrdU.Rabbit models of ACLF were created by administration of D-Gal following CCl 4-induced cirrhosis.One day after administration of D-Gal,rabbits of the ACLF/ADSCs group(n=15) were received ADSCs transplantation,while those in the ACLF/saline group(n=15) were treated with the same volume of saline.Biochemical parameters and histomorphological scoring were evaluated;the distribution and characteristics of transplanted ADSCs as well as the pathology of the liver were examined.RESULTS:ADSCs transplantation improved the survival rate and the liver function of rabbits with ACLF.Biochemical parameters of the ACLF/ADSCs group were improved compared with those of the ACLF/saline group,and histomorphological scoring of the ACLF/ADSCs group was significantly lower than that of the ACLF/saline group.ADSCs were identified in the periportal region of the liver after cell transplantation.CONCLUSION:Xenogenic ADSCs have therapeutic efficacy in the ACLF rabbit model.BACKGROUND:Adipose-derived stem cells(ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure(ACLF) This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.METHODS:ADSCs isolated from porcine fat tissue were expanded and labeled with BrdU.Rabbit models of ACLF were created by administration of D-Gal following CCl 4-induced cirrhosis.One day after administration of D-Gal,rabbits of the ACLF/ADSCs group(n=15) were received ADSCs transplantation,while those in the ACLF/saline group(n=15) were treated with the same volume of saline.Biochemical parameters and histomorphological scoring were evaluated;the distribution and characteristics of transplanted ADSCs as well as the pathology of the liver were examined.RESULTS:ADSCs transplantation improved the survival rate and the liver function of rabbits with ACLF.Biochemical parameters of the ACLF/ADSCs group were improved compared with those of the ACLF/saline group,and histomorphological scoring of the ACLF/ADSCs group was significantly lower than that of the ACLF/saline group.ADSCs were identified in the periportal region of the liver after cell transplantation.CONCLUSION:Xenogenic ADSCs have therapeutic efficacy in the ACLF rabbit model.

关 键 词：adipose-derived stem cells acute-on-chronic liver failure cell transplantation 

分 类 号：R575.3[医药卫生—消化系统]