HPLC-ELSD测定呋喃二烯脂肪乳注射液中大豆油和中链甘油三酯的含量  

Determination of soybean oil and medium-chain triglycerides in furanodiene lipid emulsion injections by HPLC-ELSD

作　　者：王艳[1] 杨秀春[1] 韩晓 杨磊[1] 孙晓岩[1] 王东凯[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳沃森药物研究所,辽宁沈阳110016

出　　处：《中国药剂学杂志（网络版）》2013年第6期115-120,共6页Chinese Journal of Pharmaceutics：Online Edition

摘　　要：目的建立呋喃二烯脂肪乳注射液中大豆油(LCT)和中链甘油三酯(MCT)的含量测定方法。方法色谱柱:Platisil Silica(250 mm×4.6 mm,5μm),流动相为正己烷-异丙醇-冰乙酸(体积比98.9:1.0:0.1),检测器为蒸发光散射检测器,气体流量为3.0 L·min-1,蒸发温度为90℃。结果 5 min内完成样品测定,LCT和MCT的分离度良好(R】1.5)。LCT和MCT分别在0.10~0.30 g·L-1内具有良好的线性关系,线性方程分别为y=1.283 9x+5.816 8(r=0.998 1)和y=1.510 5x+5.4260(r=0.999 3)。LCT回收率101.0%,RSD为1.01%(n=9);MCT回收率100.2%,RSD为1.35%(n=9)。结论方法简便、快速,结果准确。Objective To establish an HPLC-ELSD method for the determination of soybean oil and medium-chain triglycerides in furanodiene lipid emulsion injections. Method The analysis was carried out on a Platisil Silica column(250 mm × 4.6 mm,5 μm). A mixture of n-hexane-isopropanol-acetic acid( V∶ V∶V = 98. 9∶1∶0. 1) was chosen as the mobile phase. ELSD detector was applied with drift tube temperature set at 90 ℃ and gas flow rate at 3.0 L·min-1. Results A good separation among soybean oil and MCT was achieved within 5 min(R>1.5). The calibration curves were linear in the concentration ranges of 0.10-0.30 g·L-1for soybean oil and for medium-chain triglycerides,respectively. The standard curve of soybean oil was y = 1.283 9x + 5.816 8(r = 0.998 1),and the standard curve of medium-chain triglycerides was y = 1.510 5x + 5.426 0(r = 0.999 3). The average recovery of soybean oil was 101.0% with RSD 1.01%(n = 9),and the average recovery of medium-chain triglycerides was 100.2% with RSD 1.35%(n = 9). Conclusion This method was found to be accurate,rapid and simple for the determination of soybean oil and medium-chain triglycerides.

关 键 词：药剂学 脂肪乳 蒸发光散射法 大豆油 中链甘油三酯 

分 类 号：R927[医药卫生—药学]